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Label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region define stem cells in the sweat gland.
- Source :
-
PloS one [PLoS One] 2013 Sep 18; Vol. 8 (9), pp. e74174. Date of Electronic Publication: 2013 Sep 18 (Print Publication: 2013). - Publication Year :
- 2013
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Abstract
- Slow cycling is a common feature shared among several stem cells (SCs) identified in adult tissues including hair follicle and cornea. Recently, existence of unipotent SCs in basal and lumenal layers of sweat gland (SG) has been described and label retaining cells (LRCs) have also been localized in SGs; however, whether these LRCs possess SCs characteristic has not been investigated further. Here, we used a H2BGFP LRCs system for in vivo detection of infrequently dividing cells. This system allowed us to specifically localize and isolate SCs with label-retention and myoepithelial characteristics restricted to the SG proximal acinar region. Using an alternative genetic approach, we demonstrated that SG LRCs expressed keratin 15 (K15) in the acinar region and lineage tracing determined that K15 labeled cells contributed long term to the SG structure but not to epidermal homeostasis. Surprisingly, wound healing experiments did not activate proximal acinar SG cells to participate in epidermal healing. Instead, predominantly non-LRCs in the SG duct actively divided, whereas the majority of SG LRCs remained quiescent. However, when we further challenged the system under more favorable isolated wound healing conditions, we were able to trigger normally quiescent acinar LRCs to trans-differentiate into the epidermis and adopt its long term fate. In addition, dissociated SG cells were able to regenerate SGs and, surprisingly, hair follicles demonstrating their in vivo plasticity. By determining the gene expression profile of isolated SG LRCs and non-LRCs in vivo, we identified several Bone Morphogenetic Protein (BMP) pathway genes to be up-regulated and confirmed a functional requirement for BMP receptor 1A (BMPR1A)-mediated signaling in SG formation. Our data highlight the existence of SG stem cells (SGSCs) and their primary importance in SG homeostasis. It also emphasizes SGSCs as an alternative source of cells in wound healing and their plasticity for regenerating different skin appendages.
- Subjects :
- Animals
Bone Morphogenetic Protein Receptors, Type I genetics
Bone Morphogenetic Protein Receptors, Type I metabolism
Bone Morphogenetic Proteins genetics
Bone Morphogenetic Proteins metabolism
Cell Differentiation
Cell Lineage genetics
Cell Proliferation
Epidermis metabolism
Epithelial Cells metabolism
Gene Expression Regulation
Genes, Reporter
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Hair Follicle metabolism
Keratin-15 genetics
Keratin-15 metabolism
Mice
Mice, Nude
Mice, Transgenic
Regeneration
Signal Transduction
Stem Cells metabolism
Sweat Glands metabolism
Epidermal Cells
Epithelial Cells cytology
Hair Follicle cytology
Stem Cells cytology
Sweat Glands cytology
Wound Healing genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24058524
- Full Text :
- https://doi.org/10.1371/journal.pone.0074174