Dual expression of hTERT and VEGF prolongs life span and enhances angiogenic ability of aged BMSCs.

Authors :: Tang H
Xiang Y
Jiang X
Ke Y
Xiao Z
Guo Y
Wang Q
Du M
Qin L
Zou Y
Cai Y
Chen Z
Xu R
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2013 Nov 01; Vol. 440 (4), pp. 502-8. Date of Electronic Publication: 2013 Sep 18.
Publication Year :: 2013
Abstract: Previous studies have confirmed the therapeutic effects of bone marrow stromal cells (BMSCs) transplantation on cerebral ischemia. However, the proliferative, differentiative, and homing capacity of BMSC from the elderly are significantly reduced, especially after several passages expansion in vitro. In this study, by introducing lentivirus-mediated hTERT and VEGF genes to modify human BMSCs from aged donors, we observed extended lifespan, promoted angiogenic capacity while less enhanced tumorigenicity of the genetically engineering BMSCs. These results therefore suggest that the modification of aged BMSCs by dual expression of hTERT and VEGF may be used for autologous cell replacement for ischemic cerebrovascular disease in elderly patients.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)

Subjects :: Aged
Bone Marrow Cells cytology
Bone Marrow Transplantation
Cell Engineering
Genetic Engineering
Humans
Stroke therapy
Stromal Cells cytology
Stromal Cells physiology
Telomerase genetics
Vascular Endothelial Growth Factor A genetics
Bone Marrow Cells physiology
Cellular Senescence
Neovascularization, Physiologic
Telomerase biosynthesis
Vascular Endothelial Growth Factor A biosynthesis

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