Back to Search
Start Over
In vivo T-box transcription factor profiling reveals joint regulation of embryonic neuromesodermal bipotency.
- Source :
-
Cell reports [Cell Rep] 2013 Sep 26; Vol. 4 (6), pp. 1185-96. Date of Electronic Publication: 2013 Sep 19. - Publication Year :
- 2013
-
Abstract
- The design of effective cell replacement therapies requires detailed knowledge of how embryonic stem cells form primary tissues, such as mesoderm or neurectoderm that later become skeletal muscle or nervous system. Members of the T-box transcription factor family are key in the formation of these primary tissues, but their underlying molecular activities are poorly understood. Here, we define in vivo genome-wide regulatory inputs of the T-box proteins Brachyury, Eomesodermin, and VegT, which together maintain neuromesodermal stem cells and determine their bipotential fates in frog embryos. These T-box proteins are all recruited to the same genomic recognition sites, from where they activate genes involved in stem cell maintenance and mesoderm formation while repressing neurogenic genes. Consequently, their loss causes embryos to form an oversized neural tube with no mesodermal derivatives. This collaboration between T-box family members thus ensures the continuous formation of correctly proportioned neural and mesodermal tissues in vertebrate embryos during axial elongation.<br /> (Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
DNA genetics
DNA metabolism
Embryonic Stem Cells cytology
Embryonic Stem Cells metabolism
Mesoderm cytology
Mesoderm metabolism
Neural Tube cytology
Neural Tube metabolism
Neurons cytology
Neurons metabolism
T-Box Domain Proteins genetics
Xenopus
Embryonic Development physiology
T-Box Domain Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 4
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 24055059
- Full Text :
- https://doi.org/10.1016/j.celrep.2013.08.012