Back to Search
Start Over
Sdc2 and Tbx16 regulate Fgf2-dependent epithelial cell morphogenesis in the ciliated organ of asymmetry.
- Source :
-
Development (Cambridge, England) [Development] 2013 Oct; Vol. 140 (19), pp. 4102-9. - Publication Year :
- 2013
-
Abstract
- Heparan sulfate proteoglycans (HSPGs) control many cellular processes and have been implicated in the regulation of left-right (LR) development by as yet unknown mechanisms. Using lineage-targeted knockdowns, we found that the transmembrane HSPG Syndecan 2 (Sdc2) regulates LR patterning through cell-autonomous functions in the zebrafish ciliated organ of asymmetry, Kupffer's vesicle (KV), including regulation of cell proliferation and adhesion, cilia length and asymmetric fluid flow. Exploring downstream pathways, we found that the cell signaling ligand Fgf2 is exclusively expressed in KV cell lineages, and is dependent on Sdc2 and the transcription factor Tbx16. Strikingly, Fgf2 controls KV morphogenesis but not KV cilia length, and KV morphogenesis in sdc2 morphants can be rescued by expression of fgf2 mRNA. Through an Fgf2-independent pathway, Sdc2 and Tbx16 also control KV ciliogenesis. Our results uncover a novel Sdc2-Tbx16-Fgf2 pathway that regulates epithelial cell morphogenesis.
- Subjects :
- Animals
Cell Differentiation genetics
Cell Differentiation physiology
Epithelial Cells metabolism
Fibroblast Growth Factor 2 genetics
Immunohistochemistry
In Situ Hybridization
Syndecan-2 genetics
T-Box Domain Proteins genetics
Zebrafish metabolism
Zebrafish Proteins genetics
Cilia metabolism
Embryo, Nonmammalian metabolism
Epithelial Cells cytology
Fibroblast Growth Factor 2 metabolism
Syndecan-2 metabolism
T-Box Domain Proteins metabolism
Zebrafish embryology
Zebrafish Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 140
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 24046323
- Full Text :
- https://doi.org/10.1242/dev.096933