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Role of the line probe assay INNO-LiPA HBV DR and ultradeep pyrosequencing in detecting resistance mutations to nucleoside/nucleotide analogues in viral samples isolated from chronic hepatitis B patients.
- Source :
-
The Journal of general virology [J Gen Virol] 2013 Dec; Vol. 94 (Pt 12), pp. 2729-2738. Date of Electronic Publication: 2013 Sep 17. - Publication Year :
- 2013
-
Abstract
- Despite the effectiveness of nucleoside/nucleotide analogues in the treatment of chronic hepatitis B (CHB), their long-term administration is associated with the emergence of resistant hepatitis B virus (HBV) mutants. In this study, mutations resulting in antiviral resistance in HBV DNA samples isolated from 23 CHB patients (nine treatment naïve and 14 treated previously) were studied using a line probe assay (INNO-LiPA HBV DR; Innogenetics) and ultradeep pyrosequencing (UDPS) methods. Whilst the INNO-LiPA HBV DR showed no resistance mutations in HBV DNA samples from treatment-naive patients, mutations mediating lamivudine resistance were detected in three samples by UDPS. Among patients who were treated previously, 19 mutations were detected in eight samples using the INNO-LiPA HBV DR and 29 mutations were detected in 12 samples using UDPS. All mutations detected by the INNO-LiPA HBV DR were also detected by UDPS. There were no mutations that could be detected by INNO-LiPA HBV DR but not by UDPS. A total of ten mutations were detected by UDPS but not by INNO-LiPA HBV DR, and the mean frequency of these mutations was 14.7 %. It was concluded that, although INNO-LiPA HBV DR is a sensitive and practical method commonly used for the detection of resistance mutations in HBV infection, UDPS may significantly increase the detection rate of genotypic resistance in HBV at an early stage.
- Subjects :
- Adult
Female
Genotype
Hepatitis B virus enzymology
Hepatitis B virus genetics
Hepatitis B, Chronic drug therapy
Hepatitis B, Chronic virology
Humans
Lamivudine pharmacology
Male
Nucleosides pharmacology
Nucleotides pharmacology
Reverse Transcriptase Inhibitors chemistry
Sensitivity and Specificity
Sequence Analysis, DNA
Antiviral Agents pharmacology
Drug Resistance, Viral genetics
Hepatitis B virus drug effects
Molecular Diagnostic Techniques methods
Mutation
RNA-Directed DNA Polymerase genetics
Reverse Transcriptase Inhibitors pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2099
- Volume :
- 94
- Issue :
- Pt 12
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 24045109
- Full Text :
- https://doi.org/10.1099/vir.0.053041-0