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Fate of pup inside the Mycobacterium proteasome studied by in-cell NMR.
- Source :
-
PloS one [PLoS One] 2013 Sep 10; Vol. 8 (9), pp. e74576. Date of Electronic Publication: 2013 Sep 10 (Print Publication: 2013). - Publication Year :
- 2013
-
Abstract
- The Mycobacterium tuberculosis proteasome is required for maximum virulence and to resist killing by the host immune system. The prokaryotic ubiquitin-like protein, Pup-GGE, targets proteins for proteasome-mediated degradation. We demonstrate that Pup-GGQ, a precursor of Pup-GGE, is not a substrate for proteasomal degradation. Using STINT-NMR, an in-cell NMR technique, we studied the interactions between Pup-GGQ, mycobacterial proteasomal ATPase, Mpa, and Mtb proteasome core particle (CP) inside a living cell at amino acid residue resolution. We showed that under in-cell conditions, in the absence of the proteasome CP, Pup-GGQ interacts with Mpa only weakly, primarily through its C-terminal region. When Mpa and non-stoichiometric amounts of proteasome CP are present, both the N-terminal and C-terminal regions of Pup-GGQ bind strongly to Mpa. This suggests a mechanism by which transient binding of Mpa to the proteasome CP controls the fate of Pup.
- Subjects :
- Adenosine Triphosphatases chemistry
Adenosine Triphosphatases genetics
Amino Acid Sequence
Bacterial Proteins chemistry
Bacterial Proteins genetics
Binding Sites
Escherichia coli genetics
Escherichia coli metabolism
Models, Molecular
Molecular Sequence Data
Mycobacterium smegmatis genetics
Mycobacterium smegmatis metabolism
Mycobacterium tuberculosis genetics
Nuclear Magnetic Resonance, Biomolecular methods
Proteasome Endopeptidase Complex genetics
Protein Binding
Protein Interaction Domains and Motifs
Protein Isoforms chemistry
Protein Isoforms genetics
Protein Isoforms metabolism
Protein Precursors chemistry
Protein Precursors genetics
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Ubiquitins chemistry
Ubiquitins genetics
Adenosine Triphosphatases metabolism
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial
Mycobacterium tuberculosis metabolism
Proteasome Endopeptidase Complex metabolism
Protein Precursors metabolism
Ubiquitins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 24040288
- Full Text :
- https://doi.org/10.1371/journal.pone.0074576