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High nasal carriage of methicillin-resistant Staphylococcus aureus among healthy Tanzanian under-5 children.
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2014 Feb; Vol. 20 (1), pp. 82-8. Date of Electronic Publication: 2013 Sep 13. - Publication Year :
- 2014
-
Abstract
- This study aimed to determine the prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) carriage, risk factors of colonization and antimicrobial susceptibility patterns of S. aureus strains. The study was conducted at the Muhimbili National Hospital in Dar es Salaam, Tanzania. Nasal swabs were obtained from children and S. aureus was isolated and identified using conventional culture methods. MRSA was screened and confirmed using the cefoxitin disk and multiplex real-time polymerase chain reaction, respectively. Antibiotic susceptibility was performed using the Kirby-Bauer disk diffusion method. MRSA isolates were further characterized by pulsed field gel electrophoresis (PFGE) profiling. Of 285 children included in the study, S. aureus was detected in 114 (40%). Of the 114 isolates, 12 (10.5%) were MRSA. PFGE results showed that these MRSA isolates are epidemiologically unrelated. Resistance of all S. aureus to trimethoprim-sulfamethoxazole, tetracycline, gentamicin, and ciprofloxacin was 65.8%, 23.7%, 27.2%, and 4.4%, respectively. No resistance to vancomycin was found. The prevalence of inducible clindamycin resistance, constitutive clindamycin resistance, MS phenotype (resistance to erythromycin alone), and multidrug resistance was 16.7%, 1.8%, 14.0%, and 16.8%, respectively. None of the risk factors examined was found to be significant. This is the first report of S. aureus and nasal carriage of MRSA and a high rate of S. aureus carriage was found in Tanzanian under-5 children. The study findings support the need for proper health education and effective infection control measures for healthcare workers.
- Subjects :
- Anti-Bacterial Agents pharmacology
Bacterial Typing Techniques
Carrier State epidemiology
Carrier State microbiology
Cefoxitin pharmacology
Child, Preschool
Clone Cells
Drug Resistance, Multiple, Bacterial
Electrophoresis, Gel, Pulsed-Field
Female
Humans
Infant
Male
Methicillin-Resistant Staphylococcus aureus drug effects
Methicillin-Resistant Staphylococcus aureus isolation & purification
Microbial Sensitivity Tests
Nasal Cavity microbiology
Staphylococcal Infections epidemiology
Staphylococcal Infections microbiology
Tanzania epidemiology
Carrier State transmission
Methicillin-Resistant Staphylococcus aureus genetics
Staphylococcal Infections transmission
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 24033147
- Full Text :
- https://doi.org/10.1089/mdr.2013.0016