Back to Search
Start Over
Fas/FasL pathway participates in resolution of mucosal inflammatory response early during HSV-2 infection.
- Source :
-
Immunobiology [Immunobiology] 2014 Jan; Vol. 219 (1), pp. 64-77. Date of Electronic Publication: 2013 Aug 15. - Publication Year :
- 2014
-
Abstract
- Apoptotic cell death is critical for maintaining integrity of the epithelia as well as for removal of the virus infected cells. We assessed the role of Fas/FasL-dependent pathway in apoptosis of genital epithelium during HSV-2 infection using a murine model of HSV-2 infection applied to C57BL6, MRL-Fas(lpr)/J (Fas-/-) and C3-Fasl(gld)/J (FasL-/-) mice and an in vitro model of HSV-2 infection in monocyte RAW 264.7 and keratinocyte 291.03C cell cultures and peritoneal macrophages. In contrast to keratinocyte in vitro cultures, HSV-2 infection of the monocytic cell cultures led to early induction of apoptosis. HSV-2 infection of peritoneal macrophages isolated from Fas- and FasL-deficient mice showed decreased activation of apoptosis, which could be further blocked by caspase-9 inhibitor. Infection of Fas and FasL-deficient mice increased the percentage of apoptotic cells and activation of caspase-9 in the vaginal tissue in comparison to C57BL6 wild type strain. Furthermore, Fas and FasL-deficient mice showed increased infiltration of neutrophiles in the vaginal mucosal epithelium at 3 and 7 day of infection in contrast to HSV-2 infected wild-type mice. Our results show that while the Fas/FasL pathway during HSV-2 infection of the vaginal epithelium plays an important role in controlling early local inflammatory response, mitochondrial apoptotic pathway also becomes activated by the inflammatory reaction.<br /> (Copyright © 2013 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
Apoptosis genetics
Apoptosis immunology
Caspase 9 genetics
Caspase 9 immunology
Caspase 9 metabolism
Cell Line
Cells, Cultured
Epithelium metabolism
Epithelium virology
Fas Ligand Protein deficiency
Fas Ligand Protein genetics
Female
Gene Expression immunology
Herpes Genitalis genetics
Herpes Genitalis virology
Herpesvirus 2, Human physiology
Host-Pathogen Interactions immunology
Humans
Immunohistochemistry
Inflammation immunology
Inflammation virology
Keratinocytes immunology
Keratinocytes metabolism
Keratinocytes virology
Macrophages immunology
Macrophages metabolism
Macrophages virology
Mice
Mice, Inbred C57BL
Mice, Knockout
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction genetics
Time Factors
Vagina immunology
Vagina metabolism
Vagina virology
fas Receptor deficiency
fas Receptor genetics
Epithelium immunology
Fas Ligand Protein immunology
Herpes Genitalis immunology
Herpesvirus 2, Human immunology
Signal Transduction immunology
fas Receptor immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-3279
- Volume :
- 219
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Immunobiology
- Publication Type :
- Academic Journal
- Accession number :
- 24028839
- Full Text :
- https://doi.org/10.1016/j.imbio.2013.08.002