Polyethyleneimine-based transient gene expression processes for suspension-adapted HEK-293E and CHO-DG44 cells.

Authors :: Hacker DL
Kiseljak D
Rajendra Y
Thurnheer S
Baldi L
Wurm FM
Source :: Protein expression and purification [Protein Expr Purif] 2013 Nov; Vol. 92 (1), pp. 67-76. Date of Electronic Publication: 2013 Sep 08.
Publication Year :: 2013
Abstract: Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)

Subjects :: Animals
CHO Cells
Cell Culture Techniques instrumentation
Cricetinae
Cricetulus
Equipment Design
HEK293 Cells
Humans
Recombinant Proteins genetics
Viruses genetics
Drug Carriers metabolism
Genetic Vectors administration & dosage
Polyethyleneimine metabolism
Transfection methods

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