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Knockdown of maternal homeobox transcription factor SEBOX gene impaired early embryonic development in porcine parthenotes.
- Source :
-
The Journal of reproduction and development [J Reprod Dev] 2013 Dec 17; Vol. 59 (6), pp. 557-62. Date of Electronic Publication: 2013 Sep 06. - Publication Year :
- 2013
-
Abstract
- A number of germ cell-specific transcription factors essential for ovarian formation and folliculogenesis have been identified and studied. However, the role of these factors during early embryonic development has been poorly explored. In the present study, we investigated the role of SEBOX, a maternal homeobox transcription factor, during early embryonic development in porcine parthenotes. mRNA for SEBOX is preferentially expressed in oocytes, and expression persists until embryonic genome activation (EGA). Knockdown of SEBOX by siRNA disrupted early embryonic development, but not oocyte maturation. Many maternal genes essential for early embryonic development were upregulated in SEBOX-depleted embryos. Moreover, some pluripotency-associated genes, including SOX2 and NANOG, were upregulated when SEBOX was knocked down. Therefore, our data demonstrate that SEBOX is required for early embryonic development in pigs and appears to regulate the degradation of maternal transcripts and the expression of pluripotency genes.
- Subjects :
- Abattoirs
Animals
Blastocyst cytology
Cells, Cultured
Cumulus Cells cytology
Cumulus Cells metabolism
Embryo Culture Techniques
Embryo, Mammalian cytology
Female
Granulosa Cells cytology
Granulosa Cells metabolism
Homeodomain Proteins antagonists & inhibitors
Homeodomain Proteins genetics
In Vitro Oocyte Maturation Techniques
Oocytes cytology
Parthenogenesis
RNA Interference
RNA Stability
RNA, Messenger metabolism
RNA, Small Interfering
SOXB1 Transcription Factors genetics
SOXB1 Transcription Factors metabolism
Sus scrofa
Up-Regulation
Blastocyst metabolism
Ectogenesis
Embryo, Mammalian metabolism
Gene Expression Regulation, Developmental
Homeodomain Proteins metabolism
Oocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1348-4400
- Volume :
- 59
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The Journal of reproduction and development
- Publication Type :
- Academic Journal
- Accession number :
- 24018616
- Full Text :
- https://doi.org/10.1262/jrd.2013-050