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The endoplasmic reticulum is the main membrane source for biogenesis of the lytic vacuole in Arabidopsis.
- Source :
-
The Plant cell [Plant Cell] 2013 Sep; Vol. 25 (9), pp. 3434-49. Date of Electronic Publication: 2013 Sep 06. - Publication Year :
- 2013
-
Abstract
- Vacuoles are multifunctional organelles essential for the sessile lifestyle of plants. Despite their central functions in cell growth, storage, and detoxification, knowledge about mechanisms underlying their biogenesis and associated protein trafficking pathways remains limited. Here, we show that in meristematic cells of the Arabidopsis thaliana root, biogenesis of vacuoles as well as the trafficking of sterols and of two major tonoplast proteins, the vacuolar H(+)-pyrophosphatase and the vacuolar H(+)-adenosinetriphosphatase, occurs independently of endoplasmic reticulum (ER)-Golgi and post-Golgi trafficking. Instead, both pumps are found in provacuoles that structurally resemble autophagosomes but are not formed by the core autophagy machinery. Taken together, our results suggest that vacuole biogenesis and trafficking of tonoplast proteins and lipids can occur directly from the ER independent of Golgi function.
- Subjects :
- Adenosine Triphosphatases genetics
Adenosine Triphosphatases metabolism
Arabidopsis enzymology
Arabidopsis genetics
Arabidopsis ultrastructure
Arabidopsis Proteins genetics
Genes, Reporter
Golgi Apparatus metabolism
Hydrogen-Ion Concentration
Inorganic Pyrophosphatase genetics
Inorganic Pyrophosphatase metabolism
Lipid Metabolism
Meristem enzymology
Meristem genetics
Meristem physiology
Meristem ultrastructure
Plant Roots enzymology
Plant Roots genetics
Plant Roots physiology
Plant Roots ultrastructure
Plants, Genetically Modified
Protein Transport
Recombinant Fusion Proteins
Sterols metabolism
Arabidopsis physiology
Arabidopsis Proteins metabolism
Endoplasmic Reticulum metabolism
Vacuoles metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-298X
- Volume :
- 25
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 24014545
- Full Text :
- https://doi.org/10.1105/tpc.113.114827