Chemical transformation of yeast.

Authors :: Bergkessel M
Guthrie C
Source :: Methods in enzymology [Methods Enzymol] 2013; Vol. 529, pp. 311-20.
Publication Year :: 2013
Abstract: Transformation of chemically competent yeast cells is a method for introducing exogenous DNA into living cells. Typically, the DNA is either a plasmid carrying an autonomous replication sequence that allows for propagation or a linear piece of DNA to be integrated into the genome. The DNA usually also carries a marker that allows for selection of successfully transformed cells by plating on the appropriate selective media.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)