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Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype.
- Source :
-
Transfusion [Transfusion] 2014 Apr; Vol. 54 (4), pp. 1059-70. Date of Electronic Publication: 2013 Sep 04. - Publication Year :
- 2014
-
Abstract
- Background: Cultured red blood cells (cRBCs) from cord blood (CB) have been proposed as transfusion products. Whether buffy coats discarded from blood donations (adult blood [AB]) may be used to generate cRBCs for transfusion has not been investigated.<br />Study Design and Methods: Erythroid progenitor cell content and numbers and blood group antigen profiles of erythroblasts (ERYs) and cRBCs generated in human erythroid massive amplification (HEMA) culture by CB (n = 7) and AB (n = 33, three females, three males, one AB with rare blood antigens cryopreserved using CB protocols) were compared.<br />Results: Variability was observed both in progenitor cell content (twofold) and number of ERYs generated (1 log) by CB and AB in HEMA. The average progenitor cell contents of the subset of AB and CB analyzed were similar. AB generated numbers of ERYs three times lower (p < 0.01) than CB in HEMA containing fetal bovine serum but similar to CB in HEMA containing human proteins. Female AB contained two times fewer (p < 0.05) erythroid progenitor cells but generated numbers of ERYs similar to those generated by male AB. Cryopreserved AB with a rare blood group phenotype and shipped to another laboratory generated great numbers of ERYs, 90% of which matured into cRBCs. Blood group antigen expression was consistent with the donor genotype for ERYs generated both by CB and AB but concordant with that of native RBCs only for cells derived from AB.<br />Conclusion: Buffy coats from regular donors, including a donor with rare phenotypes stored under conditions established for CB, are not inferior to CB for the generation of cRBCs.<br /> (© 2013 American Association of Blood Banks.)
- Subjects :
- Adult
Blood Preservation methods
Cell Culture Techniques standards
Cells, Cultured
Erythroid Precursor Cells cytology
Erythroid Precursor Cells physiology
Female
Histocompatibility Testing
Humans
Male
Manufactured Materials standards
Phenotype
Blood Donors
Blood Preservation standards
Erythrocytes physiology
Freezing
Leukocytes, Mononuclear cytology
Leukocytes, Mononuclear physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1537-2995
- Volume :
- 54
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Transfusion
- Publication Type :
- Academic Journal
- Accession number :
- 24004289
- Full Text :
- https://doi.org/10.1111/trf.12391