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Molecular forms of cholecystokinin in rat intestine.
- Source :
-
The American journal of physiology [Am J Physiol] 1990 Sep; Vol. 259 (3 Pt 1), pp. G364-71. - Publication Year :
- 1990
-
Abstract
- The predominant immunoreactive cholecystokinin (CCK) forms in acid extracts of rat intestine eluted from reverse-phase high-performance liquid chromatography columns in the positions of CCK-8, CCK-33/39, and CCK-58. Control experiments indicated that smaller CCK forms did not arise from artifactual degradation of large CCK forms. Less than 10% of CCK-8 added to and extracted from intestine was recovered in acid; CCK-8 could not be recovered by subsequent alkaline extraction, but subsequent urea extraction yielded approximately 25% of the added peptide. This suggests that CCK binds to proteins during acid extraction and that the preponderance of large CCK forms in acid extracts is not due to inhibition of CCK degradation but results from poor extraction of small CCK forms. No evidence for a CCK-22-like peptide was found in acid or subsequent urea extracts of rat intestine, suggesting CCK posttranslational processing in adult rats is like that in humans and dogs.
- Subjects :
- Animals
Chromatography, High Pressure Liquid
Gastrins isolation & purification
Immune Sera
Male
Muscle, Smooth analysis
Protein Processing, Post-Translational
Radioimmunoassay
Rats
Rats, Inbred Strains
Cholecystokinin analogs & derivatives
Cholecystokinin isolation & purification
Intestines analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 259
- Issue :
- 3 Pt 1
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 2399981
- Full Text :
- https://doi.org/10.1152/ajpgi.1990.259.3.G364