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Novel middle-type Kenyon cells in the honeybee brain revealed by area-preferential gene expression analysis.
- Source :
-
PloS one [PLoS One] 2013 Aug 21; Vol. 8 (8), pp. e71732. Date of Electronic Publication: 2013 Aug 21 (Print Publication: 2013). - Publication Year :
- 2013
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Abstract
- The mushroom bodies (a higher center) of the honeybee (Apis mellifera L) brain were considered to comprise three types of intrinsic neurons, including large- and small-type Kenyon cells that have distinct gene expression profiles. Although previous neural activity mapping using the immediate early gene kakusei suggested that small-type Kenyon cells are mainly active in forager brains, the precise Kenyon cell types that are active in the forager brain remain to be elucidated. We searched for novel gene(s) that are expressed in an area-preferential manner in the honeybee brain. By identifying and analyzing expression of a gene that we termed mKast (middle-type Kenyon cell-preferential arrestin-related protein), we discovered novel 'middle-type Kenyon cells' that are sandwiched between large- and small-type Kenyon cells and have a gene expression profile almost complementary to those of large- and small-type Kenyon cells. Expression analysis of kakusei revealed that both small-type Kenyon cells and some middle-type Kenyon cells are active in the forager brains, suggesting their possible involvement in information processing during the foraging flight. mKast expression began after the differentiation of small- and large-type Kenyon cells during metamorphosis, suggesting that middle-type Kenyon cells differentiate by modifying some characteristics of large- and/or small-type Kenyon cells. Interestingly, CaMKII and mKast, marker genes for large- and middle-type Kenyon cells, respectively, were preferentially expressed in a distinct set of optic lobe (a visual center) neurons. Our findings suggested that it is not simply the Kenyon cell-preferential gene expression profiles, rather, a 'clustering' of neurons with similar gene expression profiles as particular Kenyon cell types that characterize the honeybee mushroom body structure.
- Subjects :
- Animals
Arrestin classification
Arrestin genetics
Brain cytology
Calcium-Calmodulin-Dependent Protein Kinase Type 2 genetics
In Situ Hybridization, Fluorescence
Insect Proteins genetics
Microscopy, Fluorescence
Mushroom Bodies cytology
Neurons cytology
Neurons metabolism
Oligonucleotide Array Sequence Analysis
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Bees genetics
Brain metabolism
Mushroom Bodies metabolism
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 8
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 23990981
- Full Text :
- https://doi.org/10.1371/journal.pone.0071732