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Genetic variability of Mycobacterium tuberculosis complex in patients with no known risk factors for MDR-TB in the North-Eastern part of Lima, Peru.
- Source :
-
BMC infectious diseases [BMC Infect Dis] 2013 Aug 28; Vol. 13, pp. 397. Date of Electronic Publication: 2013 Aug 28. - Publication Year :
- 2013
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Abstract
- Background: The aim of this study was to investigate the genetic diversity among Mycobacterium tuberculosis complex circulating in patients with no known risk factors for multi-drug resistant (MDR) tuberculosis (TB) living in a high MDR burden area and analyze the relationship between genotypes, primary drug resistance and age.<br />Methods: Samples were collected during January-July 2009. Isolates were tested for drug susceptibility to first-line drugs and were genotyped by spoligotyping and the 15-loci Mycobacterial Interspersed Repetitive Unit (MIRU15).<br />Results: Among the 199 isolates analyzed, 169 (84.9%) were identified in the SpolDB4.0 and 30 (15.1%) could not be matched to any lineage. The most prevalent lineage was Haarlem (29.6%), followed by T (15.6%), Beijing (14.1%), Latin American Mediterranean (12.6%) and U (8.5%). A few isolates belonged to the X and S clades (4.5%). Spoligotype analysis identified clustering among 148 of 169 isolates, whereas with MIRU15 all isolates were unique. Out of 197 strains; 31.5% were resistant to at least one drug, 7.5% were MDR and 22.3% showed any resistance to isoniazid.<br />Conclusion: In contrast with other Latin-American countries where LAM lineage is the most predominant, we found the spoligotype 50 from the Haarlem lineage as the most common. None of the prevailing lineages showed a significant association with age or resistance to isoniazid and/or rifampicin.
- Subjects :
- Adolescent
Adult
Aged
Antitubercular Agents pharmacology
Female
Genotype
Humans
Male
Microbial Sensitivity Tests
Middle Aged
Mycobacterium tuberculosis classification
Mycobacterium tuberculosis drug effects
Mycobacterium tuberculosis isolation & purification
Peru
Prospective Studies
Young Adult
Genetic Variation
Mycobacterium tuberculosis genetics
Tuberculosis, Multidrug-Resistant microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2334
- Volume :
- 13
- Database :
- MEDLINE
- Journal :
- BMC infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 23984854
- Full Text :
- https://doi.org/10.1186/1471-2334-13-397