Back to Search
Start Over
Bioappearance and pharmacokinetics of bioactives upon coffee consumption.
- Source :
-
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2013 Oct; Vol. 405 (26), pp. 8487-503. Date of Electronic Publication: 2013 Aug 28. - Publication Year :
- 2013
-
Abstract
- Habitual consumption of medium amounts of coffee over the whole life-span is hypothesized to reduce the risk to develop diabetes type 2 (DM2) and Alzheimer's disease (AD). To identify putative bioactive coffee-derived metabolites, first, pooled urine from coffee drinkers and non-coffee drinkers were screened by UPLC-HDMS. After statistical data analysis, trigonelline, dimethylxanthines and monomethylxanthines, and ferulic acid conjugates were identified as the major metabolites found after coffee consumption. For quantitative analysis of these markers in body fluids, targeted methods based on stable-isotope dilution and UPLC-MS/MS were developed and applied to plasma samples from a coffee intervention study (n = 13 volunteers) who consumed a single cup of caffeinated coffee brew after a 10-day washout period. Chlorogenic acid-derived metabolites were found to be separated into two groups showing different pharmacokinetic properties. The first group comprised, e.g., ferulic acid and feruloyl sulfate and showed early appearance in the plasma (~1 h). The second group contained particularly chlorogenic acid metabolites formed by the intestinal microflora, appearing late and persisting in the plasma (>6 h). Trigonelline appeared early but persisted with calculated half-life times ~5 h. The plasma levels of caffeine metabolites significantly and progressively increased 2-4 h after coffee consumption and did not reach c max within the time frame of the study. The pharmacokinetic profiles suggest that particularly trigonelline, caffeine, its metabolites, as well as late appearing dihydroferulic acid, feruloylglycine and dihydroferulic acid sulfate formed from chlorogenic acid by the intestinal microflora accumulate in the plasma due to their long half-life times during habitual consumption of several cups of coffee distributed over the day. Since some of these metabolites have been reported to show antioxidant effects in vivo, antioxidant-response-element activating potential, and neuroprotective properties, respectively, some of these key metabolites might account for the inflammation- and DM2/AD risk reducing effects reported for habitual life time consumption of coffee.
- Subjects :
- Adult
Alkaloids blood
Alkaloids urine
Caffeine blood
Caffeine urine
Chlorogenic Acid blood
Chlorogenic Acid urine
Coumaric Acids blood
Coumaric Acids urine
Female
Humans
Male
Tandem Mass Spectrometry
Xanthines blood
Xanthines urine
Young Adult
Alkaloids metabolism
Caffeine metabolism
Chlorogenic Acid metabolism
Coffee metabolism
Coumaric Acids metabolism
Xanthines metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1618-2650
- Volume :
- 405
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Analytical and bioanalytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 23982107
- Full Text :
- https://doi.org/10.1007/s00216-013-7288-0