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GSK3β inhibition and LEF1 upregulation in skeletal muscle following a bout of downhill running.
- Source :
-
The journal of physiological sciences : JPS [J Physiol Sci] 2014 Jan; Vol. 64 (1), pp. 1-11. Date of Electronic Publication: 2013 Aug 21. - Publication Year :
- 2014
-
Abstract
- Canonical Wnt signaling is important in skeletal muscle repair but has not been well characterized in response to physiological stimuli. The objective of this study was to assess the effect of downhill running (DHR) on components of Wnt signaling. Young, male C57BL/J6 mice were exposed to DHR. Muscle injury and repair (MCadherin) were measured in soleus. Gene and protein expression of Wnt3a, active β-catenin, GSK3β, and LEF1 were measured in gastrocnemius. Muscle injury increased 6 days post-DHR and MCadherin protein increased 5 days post-DHR. Total and active GSK3β protein decreased 3 days (9-fold and 3.6-fold, respectively) post-DHR. LEF1 protein increased 6 days (5-fold) post-DHR. DHR decreased GSK3β and increased LEF1 protein expression, but did not affect other components of Wnt signaling. Due to their applicability, using models of physiological stimuli such as DHR will provide significant insight into cellular mechanisms within muscle.
- Subjects :
- Animals
Cadherins metabolism
Glycogen Synthase Kinase 3 beta
Male
Mice
Mice, Inbred C57BL
Models, Animal
Time Factors
Wnt Signaling Pathway physiology
Wnt3A Protein metabolism
beta Catenin metabolism
Down-Regulation physiology
Glycogen Synthase Kinase 3 metabolism
Lymphoid Enhancer-Binding Factor 1 metabolism
Muscle, Skeletal metabolism
Physical Conditioning, Animal physiology
Running physiology
Up-Regulation physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1880-6562
- Volume :
- 64
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The journal of physiological sciences : JPS
- Publication Type :
- Academic Journal
- Accession number :
- 23963660
- Full Text :
- https://doi.org/10.1007/s12576-013-0284-5