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Synaptic protein and pan-neuronal gene expression and their regulation by Dicer-dependent mechanisms differ between neurons and neuroendocrine cells.
- Source :
-
Neural development [Neural Dev] 2013 Aug 20; Vol. 8, pp. 16. Date of Electronic Publication: 2013 Aug 20. - Publication Year :
- 2013
-
Abstract
- Background: Neurons in sympathetic ganglia and neuroendocrine cells in the adrenal medulla share not only their embryonic origin from sympathoadrenal precursors in the neural crest but also a range of functional features. These include the capacity for noradrenaline biosynthesis, vesicular storage and regulated release. Yet the regulation of neuronal properties in early neuroendocrine differentiation is a matter of debate and the developmental expression of the vesicle fusion machinery, which includes components found in both neurons and neuroendocrine cells, is not resolved.<br />Results: Analysis of synaptic protein and pan-neuronal marker mRNA expression during mouse development uncovers profound differences between sympathetic neurons and adrenal chromaffin cells, which result in qualitatively similar but quantitatively divergent transcript profiles. In sympathetic neurons embryonic upregulation of synaptic protein mRNA follows early and persistent induction of pan-neuronal marker transcripts. In adrenal chromaffin cells pan-neuronal marker expression occurs only transiently and synaptic protein messages remain at distinctly low levels throughout embryogenesis. Embryonic induction of synaptotagmin I (Syt1) in sympathetic ganglia and postnatal upregulation of synaptotagmin VII (Syt7) in adrenal medulla results in a cell type-specific difference in isoform prevalence. Dicer 1 inactivation in catecholaminergic cells reduces high neuronal synaptic protein mRNA levels but not their neuroendocrine low level expression. Pan-neuronal marker mRNAs are induced in chromaffin cells to yield a more neuron-like transcript pattern, while ultrastructure is not altered.<br />Conclusions: Our study demonstrates that remarkably different gene regulatory programs govern the expression of synaptic proteins in the neuronal and neuroendocrine branch of the sympathoadrenal system. They result in overlapping but quantitatively divergent transcript profiles. Dicer 1-dependent regulation is required to establish high neuronal mRNA levels for synaptic proteins and to maintain repression of neurofilament messages in neuroendocrine cells.
- Subjects :
- Animals
Chromaffin Cells metabolism
Chromaffin Cells ultrastructure
Chromaffin System growth & development
Chromaffin System metabolism
Ganglia, Sympathetic growth & development
Ganglia, Sympathetic metabolism
Mice
Mice, Mutant Strains
Neurofilament Proteins metabolism
RNA, Messenger metabolism
Synaptosomal-Associated Protein 25 metabolism
Synaptotagmins metabolism
rab3A GTP-Binding Protein metabolism
Chromaffin System embryology
DEAD-box RNA Helicases metabolism
Ganglia, Sympathetic embryology
Gene Expression Regulation, Developmental
Neurons metabolism
Ribonuclease III metabolism
Vesicular Transport Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1749-8104
- Volume :
- 8
- Database :
- MEDLINE
- Journal :
- Neural development
- Publication Type :
- Academic Journal
- Accession number :
- 23961995
- Full Text :
- https://doi.org/10.1186/1749-8104-8-16