Value of HIV patients with regular follow-up as in-house internal controls of flow cytometry measurement of lymphocyte subsets.

Authors :: de Carvalho Bittencourt M
Kohler C
Henard S
Rabaud C
Béné MC
Faure GC
Source :: Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2013 Sep-Oct; Vol. 84 (5), pp. 324-6. Date of Electronic Publication: 2013 Aug 08.
Publication Year :: 2013
Abstract: Background: Quality assessment in flow cytometry cannot obey the same rules as those applicable to the measurement of chemical analytes. However, regular follow-up of known patients may provide a robust in-house control of cell subsets evaluation.<br />Methods: Sequential blood samples assessed for 32 HIV patients over several years and showing good stability were retrospectively assessed to establish coefficient of variations of the percentages of CD3+, CD4+, CD8+ cells, and CD4+ absolute counts (ACs).<br />Results: Mean relative standard variations for the whole cohort were of 0.04, 0.14, 0.08, and 0.18 for CD3%, CD4%, CD8%, and CD4 ACs, respectively.<br />Discussion: In-house follow-up of regularly checked compliant patients is a good alternative to traditional and costly repeatability and reproducibility studies for the validation of routine flow cytometry. © 2013 International Clinical Cytometry Society.<br /> (Copyright © 2013 International Clinical Cytometry Society.)

Subjects :: Adult
CD4 Lymphocyte Count standards
CD4-CD8 Ratio standards
CD4-Positive T-Lymphocytes immunology
CD8-Positive T-Lymphocytes immunology
Female
Flow Cytometry standards
Follow-Up Studies
HIV immunology
HIV pathogenicity
Humans
Male
Middle Aged
T-Lymphocyte Subsets cytology
CD4-Positive T-Lymphocytes cytology
CD8-Positive T-Lymphocytes cytology
Flow Cytometry methods
HIV Infections blood

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