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Dynamin phosphorylation controls optimization of endocytosis for brief action potential bursts.
- Source :
-
ELife [Elife] 2013 Jul 30; Vol. 2, pp. e00845. Date of Electronic Publication: 2013 Jul 30. - Publication Year :
- 2013
-
Abstract
- Modulation of synaptic vesicle retrieval is considered to be potentially important in steady-state synaptic performance. Here we show that at physiological temperature endocytosis kinetics at hippocampal and cortical nerve terminals show a bi-phasic dependence on electrical activity. Endocytosis accelerates for the first 15-25 APs during bursts of action potential firing, after which it slows with increasing burst length creating an optimum stimulus for this kinetic parameter. We show that activity-dependent acceleration is only prominent at physiological temperature and that the mechanism of this modulation is based on the dephosphorylation of dynamin 1. Nerve terminals in which dynamin 1 and 3 have been replaced with dynamin 1 harboring dephospho- or phospho-mimetic mutations in the proline-rich domain eliminate the acceleration phase by either setting endocytosis at an accelerated state or a decelerated state, respectively. DOI:http://dx.doi.org/10.7554/eLife.00845.001.
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 2
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 23908769
- Full Text :
- https://doi.org/10.7554/eLife.00845