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Double-membraned liposomes sculpted by poliovirus 3AB protein.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2013 Sep 20; Vol. 288 (38), pp. 27287-27298. Date of Electronic Publication: 2013 Aug 01. - Publication Year :
- 2013
-
Abstract
- Infection with many positive-strand RNA viruses dramatically remodels cellular membranes, resulting in the accumulation of double-membraned vesicles that resemble cellular autophagosomes. In this study, a single protein encoded by poliovirus, 3AB, is shown to be sufficient to induce the formation of double-membraned liposomes via the invagination of single-membraned liposomes. Poliovirus 3AB is a 109-amino acid protein with a natively unstructured N-terminal domain. HeLa cells transduced with 3AB protein displayed intracellular membrane disruption; specifically, the formation of cytoplasmic invaginations. The ability of a single viral protein to produce structures of similar topology to cellular autophagosomes should facilitate the understanding of both cellular and viral mechanisms for membrane remodeling.
- Subjects :
- Cell Membrane genetics
Cell Membrane metabolism
HeLa Cells
Humans
Liposomes metabolism
Membrane Proteins genetics
Membrane Proteins metabolism
Protein Structure, Tertiary
Viral Nonstructural Proteins genetics
Viral Nonstructural Proteins metabolism
Cell Membrane chemistry
Liposomes chemistry
Membrane Proteins chemistry
Poliovirus
Viral Nonstructural Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1083-351X
- Volume :
- 288
- Issue :
- 38
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 23908350
- Full Text :
- https://doi.org/10.1074/jbc.M113.498899