Back to Search
Start Over
Differential protein expression profiles between Plasmodium falciparum parasites isolated from subjects presenting with pregnancy-associated malaria and uncomplicated malaria in Benin.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2013 Dec 15; Vol. 208 (12), pp. 1987-97. Date of Electronic Publication: 2013 Jul 30. - Publication Year :
- 2013
-
Abstract
- Background: Plasmodium falciparum is responsible for severe malaria, including pregnancy-associated malaria (PAM). During intra-erythrocytic maturation, the infected erythrocyte (iE) membrane is modified by insertion of parasite-derived proteins, primarily consisting of variant surface antigens such as P. falciparum erythrocyte membrane protein-1.<br />Methods: To identify new PAM-specific parasite membrane proteins, we conducted a mass spectrometry-based proteomic study and compared the protein expression profiles of 10 PAM and 10 uncomplicated malaria (UM) samples.<br />Results: We focused on the 454/1139 membrane-associated and hypothetical proteins for comparative analysis. Using filter-based feature-selection methods combined with supervised data analysis, we identified a subset of 53 proteins that distinguished PAM and UM samples. Up to 19/20 samples were correctly assigned to their respective clinical group. A hierarchical clustering analysis of these 53 proteins based on the similarity of their expression profiles revealed 2 main clusters of 40 and 13 proteins that were under- or over-expressed, respectively, in PAM.<br />Conclusions: VAR2CSA is identified and associated with PAM, validating our experimental approach. Other PAM-predictive proteins included PFI1785w, PF14&#95;0018, PFB0115w, PFF0325c, and PFA&#95;0410w. These proteomics data demonstrate the involvement of selected proteins in the pathophysiology of PAM, providing new insights for the definition of potential new targets for a vaccine against PAM.
- Subjects :
- Adult
Benin epidemiology
Child
Cluster Analysis
Female
Humans
Male
Mass Spectrometry
Membrane Proteins chemistry
Membrane Proteins classification
Parasitemia parasitology
Plasmodium falciparum pathogenicity
Pregnancy
Principal Component Analysis
Protozoan Proteins chemistry
Protozoan Proteins classification
Reproducibility of Results
Malaria, Falciparum parasitology
Membrane Proteins metabolism
Plasmodium falciparum metabolism
Pregnancy Complications, Parasitic parasitology
Protozoan Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6613
- Volume :
- 208
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 23901091
- Full Text :
- https://doi.org/10.1093/infdis/jit377