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Dicer is required for neural stem cell multipotency and lineage progression during cerebral cortex development.
- Source :
-
Neural development [Neural Dev] 2013 Jul 29; Vol. 8, pp. 14. Date of Electronic Publication: 2013 Jul 29. - Publication Year :
- 2013
-
Abstract
- Background: During cerebral cortex development, multipotent neural progenitor cells generate a variety of neuronal subtypes in a fixed temporal order. How a single neural progenitor cell generates the diversity of cortical projection neurons in a temporal sequence is not well understood. Based on their function in developmental timing in other systems, Dicer and microRNAs are potential candidate regulators of cellular pathways that control lineage progression in neural systems.<br />Results: Cortex-specific deletion of Dicer results in a marked reduction in the cellular complexity of the cortex, due to a pronounced narrowing in the range of neuronal types generated by Dicer-null cortical stem and progenitor cells. Instead of generating different classes of lamina-specific neurons in order over the 6-day period of neurogenesis, Dicer null cortical stem and progenitor cells continually produce one class of deep layer projection neuron. However, gliogenesis in the Dicer-null cerebral cortex was not delayed, despite the loss of multipotency and the failure of neuronal lineage progression.<br />Conclusions: We conclude that Dicer is required for regulating cortical stem cell multipotency with respect to neuronal diversity, without affecting the larger scale switch from neurogenesis to gliogenesis. The differences in phenotypes reported from different timings of Dicer deletion indicate that the molecular pathways regulating developmental transitions are notably dosage sensitive.
- Subjects :
- Animals
Cell Differentiation physiology
Cells, Cultured
Cerebral Cortex cytology
Cerebral Cortex metabolism
DEAD-box RNA Helicases genetics
Mice
MicroRNAs metabolism
Multipotent Stem Cells metabolism
Neural Stem Cells metabolism
Neurogenesis physiology
Neurons cytology
Neurons metabolism
Ribonuclease III genetics
Stem Cells cytology
Stem Cells metabolism
Cell Lineage physiology
Cerebral Cortex growth & development
DEAD-box RNA Helicases metabolism
Multipotent Stem Cells cytology
Neural Stem Cells cytology
Ribonuclease III metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1749-8104
- Volume :
- 8
- Database :
- MEDLINE
- Journal :
- Neural development
- Publication Type :
- Academic Journal
- Accession number :
- 23895693
- Full Text :
- https://doi.org/10.1186/1749-8104-8-14