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Electrostatic map of T7 DNA: comparative analysis of functional and electrostatic properties of T7 RNA polymerase-specific promoters.

Authors :
Kamzolova SG
Beskaravainy PM
Osypov AA
Dzhelyadin TR
Temlyakova EA
Sorokin AA
Source :
Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 2014; Vol. 32 (8), pp. 1184-92. Date of Electronic Publication: 2013 Jul 30.
Publication Year :
2014

Abstract

The entire T7 bacteriophage genome contains 39937 base pairs (Database NCBI RefSeq N1001604). Here, electrostatic potential distribution around double helical T7 DNA was calculated by Coulomb method using the computer program of Sorokin A.A. (lptolik@gmail.com). Electrostatic profiles of 17 promoters recognized by T7 phage-specific RNA polymerase were analyzed. It was shown that electrostatic profiles of all T7 RNA polymerase-specific promoters can be characterized by distinctive motifs which are specific for each promoter class. Comparative analysis of electrostatic profiles of native T7 promoters of different classes demonstrates that T7 RNA polymerase can differentiate them due to their electrostatic features.

Details

Language :
English
ISSN :
1538-0254
Volume :
32
Issue :
8
Database :
MEDLINE
Journal :
Journal of biomolecular structure & dynamics
Publication Type :
Academic Journal
Accession number :
23895582
Full Text :
https://doi.org/10.1080/07391102.2013.819298