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Pharmacokinetic and tissue distribution of paclitaxel in rabbits assayed by LC-UV after intravenous administration of its novel liposomal formulation.
- Source :
-
Biomedical chromatography : BMC [Biomed Chromatogr] 2014 Feb; Vol. 28 (2), pp. 204-12. Date of Electronic Publication: 2013 Jul 25. - Publication Year :
- 2014
-
Abstract
- A simple, rapid and sensitive LC-UV method was developed and validated for the determination of paclitaxel (PTX) in rabbit plasma and tissues. A 2 mL aliquot of acetonitrile and 10 μL ammonium acetate (pH 5.0, 6 m) as extraction agents were used to markedly increase the extraction recoveries and greatly reduce the endogenous substances. The separation was achieved on a C18 column at 30 °C using an acetonitrile-ammonium acetate buffer (pH 5.0, 0.02 m; 55:45, v/v) at a flow rate of 1.0 mL/min; UV detection was used at 227 nm. Good linearity was obtained between 0.025 and 10,000 µg/mL for plasma and between 0.025-200,000 µg/g for tissue samples (r > 0.999). The limit of detection was 6 ng/mL in plasma, 8 ng/g in heart and 12.5 ng/g in other tissues. The limit of quantitation was 25 ng/mL in plasma and heart, 125 ng/g in other tissues. The intra- and inter-day assays of precision and accuracy for all bio-samples ranged from 1.38 to 9.60% and from 83.6 to 114.5%, respectively. The extraction recoveries ranged from 70.1 to 109.5%. Samples were stable during three freeze-thaw cycles or stored in a freezer at -20 °C for 30 days. The assay method was successfully applied to a study of the pharmacokinetics and tissue distribution of novel PTX lung targeting liposomes.<br /> (Copyright © 2013 John Wiley & Sons, Ltd.)
- Subjects :
- Animals
Chromatography, High Pressure Liquid
Drug Stability
Linear Models
Liposomes chemistry
Paclitaxel chemistry
Paclitaxel isolation & purification
Rabbits
Reproducibility of Results
Sensitivity and Specificity
Tissue Distribution
Liposomes blood
Liposomes pharmacokinetics
Paclitaxel blood
Paclitaxel pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1099-0801
- Volume :
- 28
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biomedical chromatography : BMC
- Publication Type :
- Academic Journal
- Accession number :
- 23893887
- Full Text :
- https://doi.org/10.1002/bmc.3005