Back to Search Start Over

IsoDesign: a software for optimizing the design of 13C-metabolic flux analysis experiments.

Authors :
Millard P
Sokol S
Letisse F
Portais JC
Source :
Biotechnology and bioengineering [Biotechnol Bioeng] 2014 Jan; Vol. 111 (1), pp. 202-8. Date of Electronic Publication: 2013 Sep 18.
Publication Year :
2014

Abstract

The growing demand for (13) C-metabolic flux analysis ((13) C-MFA) in the field of metabolic engineering and systems biology is driving the need to rationalize expensive and time-consuming (13) C-labeling experiments. Experimental design is a key step in improving both the number of fluxes that can be calculated from a set of isotopic data and the precision of flux values. We present IsoDesign, a software that enables these parameters to be maximized by optimizing the isotopic composition of the label input. It can be applied to (13) C-MFA investigations using a broad panel of analytical tools (MS, MS/MS, (1) H NMR, (13) C NMR, etc.) individually or in combination. It includes a visualization module to intuitively select the optimal label input depending on the biological question to be addressed. Applications of IsoDesign are described, with an example of the entire (13) C-MFA workflow from the experimental design to the flux map including important practical considerations. IsoDesign makes the experimental design of (13) C-MFA experiments more accessible to a wider biological community. IsoDesign is distributed under an open source license at http://metasys.insa-toulouse.fr/software/isodes/<br /> (© 2013 Wiley Periodicals, Inc.)

Details

Language :
English
ISSN :
1097-0290
Volume :
111
Issue :
1
Database :
MEDLINE
Journal :
Biotechnology and bioengineering
Publication Type :
Academic Journal
Accession number :
23893473
Full Text :
https://doi.org/10.1002/bit.24997