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IsoDesign: a software for optimizing the design of 13C-metabolic flux analysis experiments.
- Source :
-
Biotechnology and bioengineering [Biotechnol Bioeng] 2014 Jan; Vol. 111 (1), pp. 202-8. Date of Electronic Publication: 2013 Sep 18. - Publication Year :
- 2014
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Abstract
- The growing demand for (13) C-metabolic flux analysis ((13) C-MFA) in the field of metabolic engineering and systems biology is driving the need to rationalize expensive and time-consuming (13) C-labeling experiments. Experimental design is a key step in improving both the number of fluxes that can be calculated from a set of isotopic data and the precision of flux values. We present IsoDesign, a software that enables these parameters to be maximized by optimizing the isotopic composition of the label input. It can be applied to (13) C-MFA investigations using a broad panel of analytical tools (MS, MS/MS, (1) H NMR, (13) C NMR, etc.) individually or in combination. It includes a visualization module to intuitively select the optimal label input depending on the biological question to be addressed. Applications of IsoDesign are described, with an example of the entire (13) C-MFA workflow from the experimental design to the flux map including important practical considerations. IsoDesign makes the experimental design of (13) C-MFA experiments more accessible to a wider biological community. IsoDesign is distributed under an open source license at http://metasys.insa-toulouse.fr/software/isodes/<br /> (© 2013 Wiley Periodicals, Inc.)
Details
- Language :
- English
- ISSN :
- 1097-0290
- Volume :
- 111
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biotechnology and bioengineering
- Publication Type :
- Academic Journal
- Accession number :
- 23893473
- Full Text :
- https://doi.org/10.1002/bit.24997