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Ageing-induced decrease in cardiac mitochondrial function in healthy rats.
- Source :
-
Canadian journal of physiology and pharmacology [Can J Physiol Pharmacol] 2013 Aug; Vol. 91 (8), pp. 593-600. Date of Electronic Publication: 2013 Jan 30. - Publication Year :
- 2013
-
Abstract
- It is widely recognized that mitochondrial dysfunction is a key component of the multifactorial process of ageing. The effects of age on individual components of mitochondrial function vary across species and strains. In this study we investigated the oxygen consumption, the mitochondrial membrane potential (Δψ), the sensitivity of mitochondrial permeability transition pore (mPTP) to calcium overload, and the production of reactive oxygen species (ROS) in heart mitochondria isolated from old compared with adult healthy Sprague-Dawley rats. Respirometry studies and Δψ measurements were performed with an Oxygraph-2k equipped with a tetraphenylphosphonium electrode. ROS production and calcium retention capacity were measured spectrofluorimetrically. Our results show an important decline for all bioenergetic parameters for both complex I and complex II supported-respiration, a decreased Δψ in mitochondria energized with complex I substrates, and an increased mitochondrial ROS production in the old compared with the adult group. Mitochondrial sensitivity to Ca²⁺-induced mPTP opening was also increased in the old compared with the adult animals. Moreover, the protective effect of cyclosporine A on mPTP opening was significantly reduced in the old group. We conclude that healthy ageing is associated with a decrease in heart mitochondria function in Sprague-Dawley rats.
- Subjects :
- Animals
Body Weight physiology
Calcium metabolism
Hydrogen Peroxide metabolism
Male
Membrane Potential, Mitochondrial physiology
Mitochondria, Heart metabolism
Mitochondria, Heart pathology
Mitochondrial Membrane Transport Proteins metabolism
Organ Size physiology
Oxygen Consumption physiology
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species metabolism
Aging metabolism
Aging pathology
Mitochondria, Heart physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1205-7541
- Volume :
- 91
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Canadian journal of physiology and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 23889593
- Full Text :
- https://doi.org/10.1139/cjpp-2012-0422