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Curcumin and silibinin inhibit telomerase expression in T47D human breast cancer cells.
- Source :
-
Asian Pacific journal of cancer prevention : APJCP [Asian Pac J Cancer Prev] 2013; Vol. 14 (6), pp. 3449-53. - Publication Year :
- 2013
-
Abstract
- Background: Telomerase has been considered as an attractive molecular target for breast cancer therapy. The main objective of this work is to assess the inhibitory effects of silibinin and curcumin, two herbal substances, on telomerase gene expression in breast cancer cells.<br />Materials and Methods: For determination of cell viability tetrazolium-based assays were conducted after 24, 48, and 72 h exposure times and expression of human telomerase reverse transcriptase gene was measured with real-time PCR.<br />Results: Each compound exerted cytotoxic effects on T47D cells and inhibited telomerase gene expression, both in a time-and dose-dependent manner. The mixture of curcumin and silibinin showed relatively more inhibitory effect on growth of T47D cells and hTERT gene expression as compared with either agent alone.<br />Conclusions: These findings suggest that cell viability along with hTERT gene expression in breast cancer cells could be reduced by curcumin and silibinin.
- Subjects :
- Antineoplastic Agents pharmacology
Antineoplastic Combined Chemotherapy Protocols
Antioxidants pharmacology
Apoptosis drug effects
Breast Neoplasms drug therapy
Breast Neoplasms pathology
Cell Proliferation drug effects
Female
Humans
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Silybin
Telomerase genetics
Telomerase metabolism
Tumor Cells, Cultured
Breast Neoplasms enzymology
Curcumin pharmacology
Gene Expression Regulation, Enzymologic drug effects
Gene Expression Regulation, Neoplastic drug effects
Silymarin pharmacology
Telomerase antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 2476-762X
- Volume :
- 14
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Asian Pacific journal of cancer prevention : APJCP
- Publication Type :
- Academic Journal
- Accession number :
- 23886126
- Full Text :
- https://doi.org/10.7314/apjcp.2013.14.6.3449