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Effects of caffeine on metabolism and mitochondria biogenesis in rhabdomyosarcoma cells compared with 2,4-dinitrophenol.

Authors :
Vaughan RA
Garcia-Smith R
Bisoffi M
Trujillo KA
Conn CA
Source :
Nutrition and metabolic insights [Nutr Metab Insights] 2012 Sep 13; Vol. 5, pp. 59-70. Date of Electronic Publication: 2012 Sep 13 (Print Publication: 2012).
Publication Year :
2012

Abstract

Purpose: This work investigated if treatment with caffeine or 2,4-dinitrophenol (DNP) induce expression of peroxisome proliferator-activated receptor coactivator 1 alpha (PGC-1α) and increase both mitochondrial biosynthesis and metabolism in skeletal muscle.<br />Methods: Human rhabdomyosarcoma cells were treated with either ethanol control (0.1% final concentration) caffeine, or DNP at 250 or 500 μM for 16 or 24 hours. PGC-1α RNA levels were determined using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). PGC-1α protein and mitochondrial content was determined using flow cytometry and immunohistochemistry. Metabolism was determined by quantification of extracellular acidification rate and oxygen consumption rate.<br />Results: Treatment with either caffeine or DNP induced PGC-1α RNA and protein as well as mitochondrial content compared with control. Treatment with caffeine and DNP also significantly increased oxidative metabolism and total metabolic rate compared with control. Caffeine similarly increased metabolism and mitochondrial content compared with DNP.<br />Conclusion: This work identified that both caffeine and DNP significantly induce PGC-1α, and increase both metabolism and mitochondrial content in skeletal muscle.

Details

Language :
English
ISSN :
1178-6388
Volume :
5
Database :
MEDLINE
Journal :
Nutrition and metabolic insights
Publication Type :
Academic Journal
Accession number :
23882149
Full Text :
https://doi.org/10.4137/NMI.S10233