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Two thioredoxin-superfamily members from sea bass (Dicentrarchus labrax, L.): characterization of PDI (PDIA1) and ERp57 (PDIA3).
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2013 Oct; Vol. 35 (4), pp. 1163-75. Date of Electronic Publication: 2013 Jul 21. - Publication Year :
- 2013
-
Abstract
- PDI (PDIA1) and ERp57 (PDIA3), members of the PDI family and of the thioredoxin (Trx) superfamily, are multifunctional proteins with wide physiological roles and have been implicated in several pathologies. Importantly, they are both involved in the MHC class I antigen presentation pathway. This paper reports the isolation and characterization of full cDNA and genomic clones from sea bass (Dicentrarchus labrax, L.) PDI (Dila-PDI) and ERp57 (Dila-ERp57). The genes are ~12.4 and ~7.1 kb long, originating 2155 and 2173 bp transcripts and encoding 497 and 484 amino acids mature proteins, for Dila-PDI and -ERp57, respectively. The PDI gene consists of eleven exons and ERp57 of thirteen. As described in other species, both molecules are composed of four Trx-like domains (abb'a') followed by a C-terminal tail, retaining two CGHC active sites and an ER-signalling sequence, suggestive of a conserved function. Additionally, three-dimensional homology models further support Dila-PDI and Dila-ERp57 as orthologs of mammalian PDI and ERp57, respectively. Finally, high similarity is observed to their vertebrate counterparts (>69% identity), especially among the few ones from closely related teleosts (>79% identity). Hence, these results provide relevant primary data and will enable further studies to clarify the roles of PDI and ERp57 in European sea bass immunity.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Bass metabolism
Blotting, Southern veterinary
Cloning, Molecular
DNA, Complementary genetics
DNA, Complementary metabolism
Fish Proteins chemistry
Fish Proteins metabolism
Gene Dosage
Models, Molecular
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction veterinary
Protein Disulfide-Isomerases chemistry
Protein Disulfide-Isomerases metabolism
Protein Sorting Signals
Protein Structure, Secondary
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Alignment veterinary
Thioredoxins chemistry
Thioredoxins metabolism
Bass genetics
Fish Proteins genetics
Protein Disulfide-Isomerases genetics
Thioredoxins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 35
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 23880452
- Full Text :
- https://doi.org/10.1016/j.fsi.2013.07.024