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Molecular microbiological evaluation of subgingival biofilm sampling by paper point and curette.
- Source :
-
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica [APMIS] 2014 Apr; Vol. 122 (4), pp. 347-52. Date of Electronic Publication: 2013 Jul 24. - Publication Year :
- 2014
-
Abstract
- The present clinical study aimed to investigate if there are differences in microbiological outcomes dependent on the subgingival biofilm collection method. Subgingival biofilm samples were collected from the four deepest pockets (>5 mm) of 17 patients with aggressive periodontitis (AgP) and 33 patients with chronic periodontitis (CP), first by paper point and thereafter by curette. Samples obtained with the same method were pooled together from each patient and forwarded for molecular microbiological analysis by a commercially available assay (IAI Pado Test 4.5) that estimates total bacterial load and levels of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola and Aggregatibacter actinomycetemcomitans. Data analysis included frequency of detection, quantification and correlation of detection levels between the two sampling methods. P. gingivalis, T. forsythia and T. denticola were detected in >90% of the samples, and their detection levels exhibited a strong correlation between sampling methods. The detection consistency of A. actinomycetemcomitans was 56% between the two sampling methods. A. actinomycetemcomitans was more readily detected by paper point compared with curette collection with a stronger correlation between the two methods in AgP. Subgingival biofilm sampling by curette or paper point does not yield differences in the detection of the three 'red complex' species. However, A. actinomycetemcomitans was more consistently detected by means of paper point collection, which can be crucial in the decision to administer antibiotics as an adjunctive periodontal treatment.<br /> (© 2013 APMIS. Published by John Wiley & Sons Ltd.)
- Subjects :
- Aggregatibacter actinomycetemcomitans genetics
Aggregatibacter actinomycetemcomitans isolation & purification
Bacterial Load
Bacteroidetes genetics
Bacteroidetes isolation & purification
Female
Humans
Male
Molecular Diagnostic Techniques
Paper
Periodontal Pocket microbiology
Porphyromonas gingivalis genetics
Porphyromonas gingivalis isolation & purification
RNA, Bacterial analysis
RNA, Bacterial genetics
RNA, Ribosomal, 16S analysis
RNA, Ribosomal, 16S genetics
Treponema denticola genetics
Treponema denticola isolation & purification
Aggressive Periodontitis microbiology
Bacteriological Techniques instrumentation
Biofilms
Chronic Periodontitis microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0463
- Volume :
- 122
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
- Publication Type :
- Academic Journal
- Accession number :
- 23879704
- Full Text :
- https://doi.org/10.1111/apm.12151