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Superovulation induces defective methylation in line-1 retrotransposon elements in blastocyst.
- Source :
-
Reproductive biology and endocrinology : RB&E [Reprod Biol Endocrinol] 2013 Jul 18; Vol. 11, pp. 69. Date of Electronic Publication: 2013 Jul 18. - Publication Year :
- 2013
-
Abstract
- Background: Series of epigenetic events happen during preimplantation development. Therefore assistant reproduction techniques (ART) have the potential to disrupt epigenetic regulation during embryo development. The purpose of this study was to investigate whether defects in methylation patterns in blastocyst due to superovulation originate from abnormal expression of Dnmts.<br />Methods: Low- (6 IU) and high- (10 IU) dosage of PMSG was used to stimulate the female mice. The metaphase II(MII) oocytes, zygotes and blastocyst stage embryos were collected. Global methylation and methylation at H3K9 in zygote, and methylation at repeated sequence Line 1 and IAP in blastocysts were assayed. In addition, expression of Dnmts was examined in oocytes and zygotes.<br />Results: Global DNA methylation and methylation at H3K9 in zygotes derived from females after low- or high-dosage hormone treatment were unaltered compared to that in controls. Moreover, DNA methylation at IAP in blastocysts was also unaffected, regardless of hormone dosage. In contrast, methylation at Line1 decreased when high-dose hormone was administered. Unexpectedly, expression of Dnmt3a, Dnmt3b, Dnmt3L as well as maintenance Dnmt1o in oocytes and zygotes was not disrupted.<br />Conclusions: The results suggest that defects in embryonic methylation patterns do not originate from the disruption of Dnmt expression.
- Subjects :
- Animals
Blastocyst cytology
Blastocyst drug effects
Chorionic Gonadotropin pharmacology
DNA (Cytosine-5-)-Methyltransferase 1
DNA (Cytosine-5-)-Methyltransferases genetics
DNA Methyltransferase 3A
Female
Gene Expression Regulation, Developmental
Gonadotropins, Equine pharmacology
Histones metabolism
Horses
Humans
Male
Methylation
Mice
Mice, Inbred ICR
Ovary cytology
Ovary drug effects
Ovary metabolism
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
DNA Methyltransferase 3B
Blastocyst metabolism
DNA Methylation
Long Interspersed Nucleotide Elements genetics
Superovulation
Subjects
Details
- Language :
- English
- ISSN :
- 1477-7827
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- Reproductive biology and endocrinology : RB&E
- Publication Type :
- Academic Journal
- Accession number :
- 23866265
- Full Text :
- https://doi.org/10.1186/1477-7827-11-69