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Plant Defensin type 1 (PDF1): protein promiscuity and expression variation within the Arabidopsis genus shed light on zinc tolerance acquisition in Arabidopsis halleri.
- Source :
-
The New phytologist [New Phytol] 2013 Nov; Vol. 200 (3), pp. 820-833. Date of Electronic Publication: 2013 Jul 19. - Publication Year :
- 2013
-
Abstract
- Plant defensins are recognized for their antifungal properties. However, a few type 1 defensins (PDF1s) were identified for their cellular zinc (Zn) tolerance properties after a study of the metal extremophile Arabidopsis halleri. In order to investigate whether different paralogues would display specialized functions, the A. halleri PDF1 family was characterized at the functional and genomic levels. Eleven PDF1s were isolated from A. halleri. Their ability to provide Zn tolerance in yeast cells, their activity against Fusarium oxysporum f. sp. melonii, and their level of expression in planta were compared with those of the seven A. thaliana PDF1s. The genomic organization of the PDF1 family was comparatively analysed within the Arabidopsis genus. AhPDF1s and AtPDF1s were able to confer Zn tolerance and AhPDF1s also displayed antifungal activity. PDF1 transcripts were constitutively more abundant in A. halleri than in A. thaliana. Within the Arabidopsis genus, the PDF1 family is evolutionarily dynamic, in terms of gain and loss of gene copy. Arabidopsis halleri PDF1s display no superior abilities to provide Zn tolerance. A constitutive increase in AhPDF1 transcript accumulation is proposed to be an evolutionary innovation co-opting the promiscuous PDF1 protein for its contribution to Zn tolerance in A. halleri.<br /> (No claim to original French government works. New Phytologist © 2013 New Phytologist Trust.)
- Subjects :
- Amino Acid Sequence
Arabidopsis metabolism
Arabidopsis Proteins isolation & purification
Arabidopsis Proteins metabolism
Arabidopsis Proteins pharmacology
Defensins metabolism
Disease Resistance genetics
Evolution, Molecular
Fusarium drug effects
Genes, Plant
Genome, Plant
Molecular Sequence Data
Stress, Physiological genetics
Yeasts
Adaptation, Physiological genetics
Arabidopsis genetics
Arabidopsis Proteins genetics
Defensins genetics
Gene Expression
Gene Expression Regulation, Plant
Zinc metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 200
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 23865749
- Full Text :
- https://doi.org/10.1111/nph.12396