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Obesity-related metabolomic analysis of human subjects in black soybean peptide intervention study by ultraperformance liquid chromatography and quadrupole-time-of-flight mass spectrometry.
- Source :
-
Journal of obesity [J Obes] 2013; Vol. 2013, pp. 874981. Date of Electronic Publication: 2013 Jun 04. - Publication Year :
- 2013
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Abstract
- The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP) for 12 weeks. This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated. Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and the data were analyzed using partial least-squares discriminate analysis (PLS-DA) score plots. Body mass index and percent body fat of the test group were reduced. Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, l-aspartyl-l-phenylalanine, and lysophosphatidyl cholines (lysoPCs) (C18:1, C18:2, C20:1, and C20:4) showed significant increases. Levels of l-proline, valine, l-leucine/isoleucine, hypoxanthine, glutamine, l-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0) were significantly decreased. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects' blood. Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood.
- Subjects :
- Adult
Aged
Biomarkers blood
Discriminant Analysis
Female
Humans
Least-Squares Analysis
Male
Middle Aged
Obesity blood
Obesity diagnosis
Time Factors
Treatment Outcome
Weight Loss drug effects
Young Adult
Chromatography, Liquid
Dietary Supplements
Metabolomics methods
Obesity drug therapy
Plant Proteins, Dietary therapeutic use
Glycine max
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Subjects
Details
- Language :
- English
- ISSN :
- 2090-0716
- Volume :
- 2013
- Database :
- MEDLINE
- Journal :
- Journal of obesity
- Publication Type :
- Academic Journal
- Accession number :
- 23862058
- Full Text :
- https://doi.org/10.1155/2013/874981