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C-reactive protein inhibits lymphangiogenesis and resultant lymph node metastasis of squamous cell carcinoma in mice.
- Source :
-
Surgery [Surgery] 2013 Nov; Vol. 154 (5), pp. 1087-92. Date of Electronic Publication: 2013 Jul 13. - Publication Year :
- 2013
-
Abstract
- Background: Lymph node involvement is the most important prognostic factor in many solid cancers. Recently, we found that patients with esophageal and lung cancer carrying the C-reactive protein (CRP) 1846T/T genotype, which is associated with lower serum CRP levels, are more likely to have lymph node metastasis. We hypothesized that host CRP directly inhibits lymph node metastasis.<br />Methods: We inoculated NR-S1M metastatic cells subcutaneously into the backs of C3H/HeN mice. Concurrently, 1 μg of recombinant mouse CRP or phosphate-buffered saline was injected subcutaneously every 3 days for 5 weeks, after which the mice were killed for evaluation. We evaluated lymph node metastasis and lymphangiogenesis in the implanted tumor by using immunohistochemical analysis with anti-pancytokeratin and antilymphatic vessel endothelial hyaluronan receptor-1 antibodies.<br />Results: There was no substantial difference in tumor size between the 2 groups but the lymph nodes were smaller in the CRP group than the control group (P < .044). Immunohistochemical analysis confirmed inguinal lymph node metastasis in 70% (14/20) of control mice, but in only 30% (3/10) of mice in the CRP group. Moreover, the metastatic area within lymph nodes was less in the CRP group (P < .042) and tumoral lymphangiogenesis was decreased in the CRP group (P < .037).<br />Conclusion: CRP appears to inhibit tumoral lymphangiogenesis and lymph node metastasis in mice. These findings suggest that by inhibiting lymph node metastasis, CPR may have therapeutic potential for use against cancer.<br /> (Copyright © 2013 Mosby, Inc. All rights reserved.)
- Subjects :
- Animals
C-Reactive Protein therapeutic use
Carcinoma, Squamous Cell pathology
Carcinoma, Squamous Cell physiopathology
Cell Line, Tumor
Female
Lymph Nodes pathology
Lymphatic Metastasis pathology
Lymphatic Metastasis physiopathology
Lymphatic Metastasis prevention & control
Mice
Mice, Inbred C3H
C-Reactive Protein physiology
Carcinoma, Squamous Cell drug therapy
Lymphangiogenesis physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1532-7361
- Volume :
- 154
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 23859309
- Full Text :
- https://doi.org/10.1016/j.surg.2013.04.016