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[Construction of cell surface display system in lactic acid bacteria by using ice nucleation protein].
- Source :
-
Wei sheng wu xue bao = Acta microbiologica Sinica [Wei Sheng Wu Xue Bao] 2013 Apr 04; Vol. 53 (4), pp. 397-402. - Publication Year :
- 2013
-
Abstract
- Objective: The gene of ice nucleation protein (INP), an outer membrane of Pseudomonas syringae was tested to display foreign proteins on the surface of Lactococcus lactis.<br />Methods: Plasmids pHZ101 and pHZ102 were constructed using gfp (Green Fluorescence Protein) as the reporter gene and N-terminal and NC-terminal of inp as the anchoring units. Plasmids pHZ101 and pHZ102 were subsequently transformed into Escherichia coli JM109 and Lactococcus lactis MG1363.<br />Results: Fluorescence microscope shows that green fluorescence was observed in both recombinant E. coli and L. lactis strains. Western blot indicated that GFP was expressed in both recombinant E. coli and L. lactis strains. INPN-GFP was mostly trapped in cytoplastic fraction while INPNC-GFP was mainly targeted on the cell membrane of L. lactis.<br />Conclusion: The results suggest a new way to construct cell surface display system of lactic acid bacteria by using ice nucleation protein.
- Subjects :
- Escherichia coli genetics
Escherichia coli metabolism
Green Fluorescent Proteins genetics
Green Fluorescent Proteins metabolism
Plasmids genetics
Pseudomonas syringae genetics
Pseudomonas syringae metabolism
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins metabolism
Bacterial Outer Membrane Proteins genetics
Bacterial Outer Membrane Proteins metabolism
Cell Membrane genetics
Cell Membrane metabolism
Lactic Acid metabolism
Lactococcus lactis genetics
Lactococcus lactis metabolism
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0001-6209
- Volume :
- 53
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Wei sheng wu xue bao = Acta microbiologica Sinica
- Publication Type :
- Academic Journal
- Accession number :
- 23858715