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BCL10 expression and localization in ocular adnexa MALT lymphomas: a comparative cytogenetic and immunohistochemical study.
- Source :
-
Histology and histopathology [Histol Histopathol] 2014 Jan; Vol. 29 (1), pp. 77-87. Date of Electronic Publication: 2013 Jul 12. - Publication Year :
- 2014
-
Abstract
- T(1;14) (p22;q32) involving BCL10 and IGH genes is a rare but recurrent chromosomal aberration in MALT-type lymphoma. It is rarely described in ocular adnexa B cell lymphomas, although nuclear BCL10 shuttling seems to be critical for disease progression in this district. We have evaluated the translocations MALT lymphoma-related in a series of 45 ocular adnexa cases, focusing in particular on their relation with BCL10 expression and its cellular topographic distribution. A prognostic tissue microarray (TMA) with ocular adnexa MALT lymphomas was designed. A study of BCL10 expression and its topographic distribution was performed through immunohistochemistry. In addition the assessment of t(14;18) (q32;q21), t(1;14) (p22;q32) and t(11;18) (q21;q21) was determined by Fluorescent In Situ Hybridization (FISH). Our series revealed t(14;18) (q32;q21) in 6/43 cases (14,3%). t(1;14) (p22;q32), never described in ocular adnexa MALT lymphomas, was observed in 3/31 (9,7%), two of which exhibited the gain of 3' upstream BCL10 gene signal (4%), whereas no case showed t(11;18) (q21;q21). Moreover, BCL10 expression was observed in 18/45 cases. In particular its nuclear expression was revealed in 12/45 cases, cytoplasmic expression in 5/45 and both cytoplasmic and nuclear expression in 1/45. Statistical analysis demonstrated that while BCL10 cytoplasmic expression is significantly related to the presence of the investigated chromosomal aberrations, in particular with t(14;18) (q32;q21), BCL10 nuclear shuttling does not show any correlation with these translocations. Our data support that BCL10 nuclear distribution is neither related to BCL10 rearrangement nor to other known translocations.
- Subjects :
- Adaptor Proteins, Signal Transducing biosynthesis
Adult
Aged
B-Cell CLL-Lymphoma 10 Protein
Biomarkers, Tumor analysis
Cytogenetic Analysis
Eye Neoplasms pathology
Female
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Lymphoma, B-Cell, Marginal Zone pathology
Male
Middle Aged
Tissue Array Analysis
Translocation, Genetic
Eye Neoplasms genetics
Eye Neoplasms metabolism
Lymphoma, B-Cell, Marginal Zone genetics
Lymphoma, B-Cell, Marginal Zone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1699-5848
- Volume :
- 29
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Histology and histopathology
- Publication Type :
- Academic Journal
- Accession number :
- 23846624
- Full Text :
- https://doi.org/10.14670/HH-29.77