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Synthesis, characterization, and DNA binding, photocleavage, cytotoxicity, cellular uptake, apoptosis, and on-off light switching studies of Ru(II) mixed-ligand complexes containing 7-fluorodipyrido[3,2-a:2',3'-c]phenazine.
- Source :
-
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry [J Biol Inorg Chem] 2013 Oct; Vol. 18 (7), pp. 751-66. Date of Electronic Publication: 2013 Jul 12. - Publication Year :
- 2013
-
Abstract
- Four new ruthenium(II) polypyridyl complexes-[Ru(phen)2(7-F-dppz)](2+) (7-F-dppz is 7-fluorodipyrido[3,2-a:2',3'-c]phenazine, phen is 1,10-phenanthroline), [Ru(bpy)2(7-F-dppz)](2+)(2) (bpy is 2,2'-bipyridine), [Ru(dmb)2(7-F-dppz)](2+) (dmb is 4,4'-dimethyl-2,2'-bipyridine), and [Ru(hdpa)2(7-F-dppz)](2+) (hdpa is 2,2'-dipyridylamine)-have been synthesized and characterized. Their DNA binding behavior has been explored by various spectroscopic titrations and viscosity measurements, which indicated that all the complexes bind to calf thymus DNA by means of intercalation with different binding strengths. The light switching properties of these complexes have been evaluated, and their antimicrobial activities have been investigated. Photoinduced DNA cleavage studies have been performed. All the complexes exhibited efficient photocleavage of pBR322 DNA on irradiation. The cytotoxicity of these complexes has been evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay with various tumor cell lines. Cellular uptake was studied by flow cytometry and confocal microscopy. Flow cytometry experiments showed that these complexes induced apoptosis of HeLa cell lines.
- Subjects :
- Anti-Infective Agents chemical synthesis
Anti-Infective Agents chemistry
Anti-Infective Agents metabolism
Anti-Infective Agents pharmacology
Antineoplastic Agents chemical synthesis
Antineoplastic Agents chemistry
Antineoplastic Agents metabolism
Antineoplastic Agents pharmacology
Biological Transport
Cell Line, Tumor
Cell Proliferation drug effects
Chemistry Techniques, Synthetic
DNA chemistry
DNA Cleavage radiation effects
Escherichia coli drug effects
Humans
Organometallic Compounds chemistry
Organometallic Compounds metabolism
Phenazines chemistry
Staphylococcus aureus drug effects
Viscosity
Apoptosis drug effects
DNA metabolism
DNA Cleavage drug effects
Light
Organometallic Compounds chemical synthesis
Organometallic Compounds pharmacology
Ruthenium chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1327
- Volume :
- 18
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 23846180
- Full Text :
- https://doi.org/10.1007/s00775-013-1018-0