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Plant microRNAs display differential 3' truncation and tailing modifications that are ARGONAUTE1 dependent and conserved across species.
- Source :
-
The Plant cell [Plant Cell] 2013 Jul; Vol. 25 (7), pp. 2417-28. Date of Electronic Publication: 2013 Jul 09. - Publication Year :
- 2013
-
Abstract
- Plant small RNAs are 3' methylated by the methyltransferase HUA1 ENHANCER1 (HEN1). In plant hen1 mutants, 3' modifications of small RNAs, including oligo-uridylation (tailing), are associated with accelerated degradation of microRNAs (miRNAs). By sequencing small RNAs of the wild type and hen1 mutants from Arabidopsis thaliana, rice (Oryza sativa), and maize (Zea mays), we found 3' truncation prior to tailing is widespread in these mutants. Moreover, the patterns of miRNA truncation and tailing differ substantially among miRNA families but are conserved across species. The same patterns are also observable in wild-type libraries from a broad range of species, only at lower abundances. ARGONAUTE (AGO1), even with defective slicer activity, can bind these truncated and tailed variants of miRNAs. An ago1 mutation in hen1 suppressed such 3' modifications, indicating that they occur while miRNAs are in association with AGO1, either during or after RNA-induced silencing complex assembly. Our results showed AGO1-bound miRNAs are actively 3' truncated and tailed, possibly reflecting the activity of cofactors acting in conserved patterns in miRNA degradation.
- Subjects :
- Arabidopsis genetics
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Argonaute Proteins metabolism
Base Sequence
Gene Expression Profiling
Gene Expression Regulation, Plant
Methylation
MicroRNAs metabolism
Mutation
Oligonucleotide Array Sequence Analysis
Oryza genetics
Oryza metabolism
Plant Proteins genetics
Plant Proteins metabolism
Protein Binding
RNA, Plant metabolism
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Nucleic Acid
Species Specificity
Zea mays genetics
Zea mays metabolism
Arabidopsis Proteins genetics
Argonaute Proteins genetics
MicroRNAs genetics
RNA, Plant genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1532-298X
- Volume :
- 25
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- The Plant cell
- Publication Type :
- Academic Journal
- Accession number :
- 23839787
- Full Text :
- https://doi.org/10.1105/tpc.113.114603