Back to Search
Start Over
A competition binding assay for determination of the inositol (1,4,5)-trisphosphate content of human leucocytes.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1990 Jul 31; Vol. 170 (2), pp. 755-62. - Publication Year :
- 1990
-
Abstract
- We developed a competition binding assay for estimation of the intracellular inositol (1,4,5)-trisphosphate (Ins(1,4,5)P3) and optimalized it for the measurement of the Ins(1,4,5)P3 content of human blood leucocytes. The present method is considerably cheaper and requires five times fewer cells than the commercial Ins(1,4,5)P3 kit. The mean Ins(1,4,5)P3 content of human blood monocytes, granulocytes, and lymphocytes amounted to 3.3 +/- 1.2 microM, 3.1 +/- 1.4 microM, and 4.6 +/- 1.5 microM, respectively. After stimulation with formyl-methionyl-leucyl-phenylalanine (f-MLP) the Ins(1,4,5)P3 content of human granulocytes and monocytes increased 2-3 times within 10 sec and then gradually decreased, returning to basal values at 60 sec. Lymphocytes did not respond to f-MLP with an increase in their Ins(1,4,5)P3 content.
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 170
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 2383266
- Full Text :
- https://doi.org/10.1016/0006-291x(90)92155-s