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Diabetes impairs synaptic plasticity in the superior cervical ganglion: possible role for BDNF and oxidative stress.
- Source :
-
Journal of molecular neuroscience : MN [J Mol Neurosci] 2013 Nov; Vol. 51 (3), pp. 763-70. Date of Electronic Publication: 2013 Jul 06. - Publication Year :
- 2013
-
Abstract
- The majority of diabetics develop serious disorders of the autonomic nervous system; however, there is no clear understanding on the causes of these complications. In this study, we examined the effect of streptozocin (STZ)-induced diabetes on activity-dependent synaptic plasticity, associated levels of brain-derived neurotrophic factor (BDNF) and antioxidant biomarkers in the rat sympathetic superior cervical ganglion. Diabetes (STZ-induced) was achieved by a single intraperitoneal injection of streptozocin (55 mg/kg).Compound action potentials were recorded from isolated ganglia before (basal) and after repetitive stimulation, or trains of paired pulses to express ganglionic long-term potentiation (gLTP) or long-term depression (gLTD). The input/output curves of ganglia from STZ-treated animals showed a marked rightward shift along most stimulus intensities, compared to those of ganglia from control animals, indicating impaired basal synaptic transmission in ganglia from STZ-induced diabetic animals. Repetitive stimulation induced robust gLTP and gLTD in ganglia isolated from control animals; the same protocols failed to induce gLTP or gLTD in ganglia from STZ-induced diabetic animals, indicating impairment of activity-dependent synaptic plasticity in these animals. Molecular analysis revealed significant reduction in the levels of BDNF and the ratio of glutathione/oxidized glutathione. Additionally, the activity of glutathione peroxidase, glutathione reductase, catalase, and the levels of thiobarbituric acid-reactive substances were increased in ganglia from STZ-treated animals. In conclusion, impaired basal synaptic transmission and synaptic plasticity are associated with reduced BDNF and altered oxidative stress biomarkers in the sympathetic ganglia from STZ-induced diabetic animals, suggesting a possible correlation of these factors with the manifestations of STZ-induced diabetes in the peripheral nervous system.
- Subjects :
- Action Potentials
Animals
Diabetes Mellitus, Experimental physiopathology
Glutathione metabolism
Male
Rats
Rats, Wistar
Superior Cervical Ganglion physiopathology
Brain-Derived Neurotrophic Factor metabolism
Diabetes Mellitus, Experimental metabolism
Long-Term Potentiation
Oxidative Stress
Superior Cervical Ganglion metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1559-1166
- Volume :
- 51
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of molecular neuroscience : MN
- Publication Type :
- Academic Journal
- Accession number :
- 23832486
- Full Text :
- https://doi.org/10.1007/s12031-013-0061-1