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Molecular cloning and partial characterization of an ovarian receptor with seven ligand binding repeats, an orthologue of low-density lipoprotein receptor, in the cutthroat trout (Oncorhynchus clarki).
- Source :
-
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2013 Oct; Vol. 166 (2), pp. 263-71. Date of Electronic Publication: 2013 Jul 01. - Publication Year :
- 2013
-
Abstract
- Teleost fish eggs contain a substantial yolk mass consisting of lipids and proteins that provides essential nutrients for embryonic and larval development. The polar lipid and protein components of the yolk are delivered to oocytes by circulating vitellogenins, however the source(s) of the neutral lipid remains unknown. We cloned a cDNA encoding an orthologue of low-density-lipoprotein receptor (LDLR) from the ovary of cutthroat trout, Oncorhynchus clarki (ct-Ldlr). Predominant expression of ct-ldlr mRNA was observed in the ovary and moderate expression was detected in intestine, gill and brain. The relative abundance of ct-ldlr transcripts was highest in early pre-vitellogenic ovaries and significantly decreased during vitellogenesis, followed by a slight increase during final maturation and in post-ovulatory follicles. In situ hybridization revealed an intense and evenly distributed localization of ct-ldlr transcripts in the ooplasm of pre-vitellogenic oocytes and these signals disappeared in vitellogenic follicles. Collectively, these results suggest that the Ldlr is involved in deposition of yolk lipids in cutthroat trout oocytes. The ct-ldlr transcripts also were detected in theca and granulosa cells, suggesting that this receptor may be involved in cholesterol uptake for ovarian steroidogenesis. This is the first report on partial characterization of an ldlr orthologue in any fish species.<br /> (© 2013.)
- Subjects :
- Amino Acid Sequence
Animals
Cloning, Molecular
Female
Fish Proteins metabolism
Gene Expression
Molecular Sequence Data
Oocytes metabolism
Organ Specificity
Ovarian Follicle cytology
Phylogeny
RNA, Messenger genetics
RNA, Messenger metabolism
Receptors, LDL metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trout metabolism
Fish Proteins genetics
Ovarian Follicle metabolism
Receptors, LDL genetics
Trout genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1531-4332
- Volume :
- 166
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
- Publication Type :
- Academic Journal
- Accession number :
- 23827521
- Full Text :
- https://doi.org/10.1016/j.cbpa.2013.06.026