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Susceptibility of BALB/c-nu/nu mice and BALB/c mice to equine herpesvirus 9 infection.
- Source :
-
Veterinary pathology [Vet Pathol] 2014 May; Vol. 51 (3), pp. 581-90. Date of Electronic Publication: 2013 Jun 26. - Publication Year :
- 2014
-
Abstract
- This study aimed to clarify the timing and infectivity of equine herpesvirus 9 (EHV-9) infection in BALB/c-nu/nu mice and their immunocompetent counterpart (BALB/c). Following intranasal inoculation with 10(5) PFU of EHV-9, specimens from 8 mice per group were collected at different times postinoculation (PI) and assessed using histopathology, immunohistochemistry for viral antigen, and quantitative real-time polymerase chain reaction for ORF30 gene expression. In BALB/c-nu/nu mice, EHV-9 antigen was abundant in olfactory epithelia of all inoculated animals, and in the olfactory bulb of 1 animal. In contrast, only 1 BALB/c mouse per time point had rhinitis, with mild to moderate immunopositivity starting from 12 to 48 h PI, followed by a gradual virus clearance at 72 h PI. Statistically, significant differences were noted in the immunohistochemistry reactions between the 2 mouse strains, indicating that BALB/c-nu/nu is more susceptible to infection. Relative expression levels of ORF30 gene in olfactory epithelia were significantly different between the 2 groups, with the exception of 12 h PI, when BALB/c-nu/nu animals showed dramatic increases in ORF30 gene expression level until 48 h PI, followed by a decline in expression level until the end of experiment. In contrast, the expression level in brains showed no differences between mouse strain except at 96 h PI. In both strains, the highest messenger RNA expression was detected at 48 h PI, followed by a decline in BALB/c mice, proving a rapid clearance of virus in BALB/c and a gradual slowing down of the increased expression levels in BALB/c-nu/nu.
- Subjects :
- Administration, Intranasal
Animals
Antigens, Viral metabolism
Cattle
Cell Line
DNA Primers genetics
Herpesviridae Infections metabolism
Immunohistochemistry veterinary
Mice
Olfactory Mucosa virology
RNA, Messenger metabolism
Real-Time Polymerase Chain Reaction
Statistics, Nonparametric
Disease Susceptibility pathology
Herpesviridae Infections veterinary
Mice, Inbred BALB C
Mice, Nude
Rodent Diseases metabolism
Rodent Diseases virology
Varicellovirus pathogenicity
Subjects
Details
- Language :
- English
- ISSN :
- 1544-2217
- Volume :
- 51
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Veterinary pathology
- Publication Type :
- Academic Journal
- Accession number :
- 23804999
- Full Text :
- https://doi.org/10.1177/0300985813493932