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Therapeutic effects of bacteriophages against Salmonella gallinarum infection in chickens.
- Source :
-
Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2013 Oct 28; Vol. 23 (10), pp. 1478-83. - Publication Year :
- 2013
-
Abstract
- In this study the isolation and characterization of three bacteriophages (ST4, L13, and SG3) infecting Salmonella gallinarum were carried out. They were further tested for their in vivo efficacy in phage therapy. All three phages belong to the Siphoviridae family with isometric heads and non-contractile tails. They have a broad host range among serovars of Salmonella enterica. The burst sizes were observed to be 1670, 80, and 28 for ST4, L13, and SG3, respectively. The in vivo efficacy of the phages was tested in chickens. Layer chickens were challenged with S. gallinarum, whereas contact chickens were cohabited without direct challenge. Each bacteriophage was orally inoculated in the form of feed additives. Mortality was observed and S. gallinarum was periodically re-isolated from the livers, spleens, and cecums of the chickens. Bacterial re-isolation from the organs and mortality decreased significantly in both challenged and contact chickens treated with the bacteriophages compared with untreated chickens serving as the control. The three bacteriophages may be effective alternatives to antibiotics for the control of fowl typhoid disease in chickens.
- Subjects :
- Animal Structures microbiology
Animals
Chickens
DNA, Viral chemistry
DNA, Viral genetics
Host Specificity
Microscopy, Electron, Transmission
Molecular Sequence Data
Poultry Diseases pathology
Salmonella Infections, Animal pathology
Salmonella Phages isolation & purification
Salmonella Phages physiology
Salmonella Phages ultrastructure
Salmonella enterica virology
Sequence Analysis, DNA
Siphoviridae growth & development
Siphoviridae isolation & purification
Siphoviridae physiology
Siphoviridae ultrastructure
Survival Analysis
Virion ultrastructure
Biological Therapy methods
Poultry Diseases microbiology
Poultry Diseases therapy
Salmonella Infections, Animal therapy
Salmonella Phages growth & development
Subjects
Details
- Language :
- English
- ISSN :
- 1738-8872
- Volume :
- 23
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 23801253
- Full Text :
- https://doi.org/10.4014/jmb.1304.04067