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Identification of calcium-transporting ATPases of Entamoeba histolytica and cellular localization of the putative SERCA.

Authors :
Martinez-Higuera A
Salas-Casas A
Calixto-Gálvez M
Chávez-Munguía B
Pérez-Ishiwara DG
Ximénez C
Rodríguez MA
Source :
Experimental parasitology [Exp Parasitol] 2013 Sep; Vol. 135 (1), pp. 79-86. Date of Electronic Publication: 2013 Jun 22.
Publication Year :
2013

Abstract

Calcium has an important role on signaling of different cellular processes in the protozoa parasite Entamoeba histolytica, including development and pathogenesis. However, the systems that control calcium responses in this parasite are incompletely understood. Calcium-ATPases (Ca(2+)-ATPases) are proteins that play an important role in calcium homeostasis by catalyzing the active efflux of this ion from cytoplasm and are essential to the correct functioning of the cell machinery. Here, we reported the identification of five E. histolytica genes encoding putative Ca(2+)-ATPases, three related to PMCA, and two related to organellar ATPases. RT-PCR assays showed that all those genes are expressed in trophozoites and specific antibodies against the SERCA-like member located this protein in a continuous cytoplasmic network, supporting the hypothesis that it corresponds to the Ca(2+)-ATPase responsible to sequester calcium in the endoplasmic reticulum of this parasite.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1090-2449
Volume :
135
Issue :
1
Database :
MEDLINE
Journal :
Experimental parasitology
Publication Type :
Academic Journal
Accession number :
23800535
Full Text :
https://doi.org/10.1016/j.exppara.2013.06.004