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Neurokinin-1 receptor, a new modulator of lymphangiogenesis in obese-asthma phenotype.
- Source :
-
Life sciences [Life Sci] 2013 Aug 06; Vol. 93 (4), pp. 169-77. Date of Electronic Publication: 2013 Jun 20. - Publication Year :
- 2013
-
Abstract
- Aims: Obesity and asthma are widely prevalent and associated disorders. Recent studies of our group revealed that Substance P (SP) is involved in pathophysiology of obese-asthma phenotype in mice through its selective NK1 receptor (NK1-R). Lymphangiogenesis is impaired in asthma and obesity, and SP activates contractile and inflammatory pathways in lymphatics. Our aim was to study whether NK1-R expression was involved in lymphangiogenesis on visceral (VAT) and subcutaneous (SAT) adipose tissues and in the lungs, in obese-allergen sensitized mice.<br />Main Methods: Diet-induced obese and ovalbumin (OVA)-sensitized Balb/c mice were treated with a selective NK1-R antagonist (CJ 12,255, Pfizer Inc., USA) or placebo. Lymphatic structures (LYVE-1+) and NK1-R expression were analyzed by immunohistochemistry. A semi-quantitative score methodology was used for NK1-R expression.<br />Key Findings: Obesity and allergen-sensitization together increased the number of LYVE-1+ lymphatics in VAT and decreased it in SAT and lungs. NK1-R was mainly expressed on adipocyte membranes of VAT, blood vessel areas of SAT, and in lung epithelium. Obesity and allergen-sensitization combined increased the expression of NK1-R in VAT, SAT and lungs. NK1-R antagonist treatment reversed the effects observed in lymphangiogenesis in those tissues.<br />Significance: The obese-asthma phenotype in mice is accompanied by increased expression of NK1-R on adipose tissues and lung epithelium, reflecting that SP released during inflammation may act directly on these tissues. Blocking NK1-R affects lymphangiogenesis, implying a role of SP, with opposite physiological consequences in VAT, and in SAT and lungs. Our results provide a clue for a novel SP role in the obese-asthma phenotype.<br /> (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Asthma pathology
Intra-Abdominal Fat metabolism
Intra-Abdominal Fat pathology
Lung metabolism
Lung pathology
Lymphatic Vessels pathology
Mice
Mice, Inbred BALB C
Obesity pathology
Phenotype
Respiratory Mucosa metabolism
Respiratory Mucosa pathology
Subcutaneous Fat, Abdominal metabolism
Subcutaneous Fat, Abdominal pathology
Asthma metabolism
Gene Expression Regulation
Lymphatic Vessels metabolism
Obesity metabolism
Receptors, Neurokinin-1 biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0631
- Volume :
- 93
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 23792204
- Full Text :
- https://doi.org/10.1016/j.lfs.2013.06.010