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Postweaning exposure to a high-fat diet is associated with alterations to the hepatic histone code in Japanese macaques.
- Source :
-
Pediatric research [Pediatr Res] 2013 Sep; Vol. 74 (3), pp. 252-8. Date of Electronic Publication: 2013 Jun 20. - Publication Year :
- 2013
-
Abstract
- Background: Expression of circadian gene, Npas2, is altered in fetal life with maternal high-fat (HF) diet exposure by virtue of alterations in the fetal histone code. We postulated that these disruptions would persist postnatally.<br />Methods: Pregnant macaques were fed a control (CTR) or HF diet and delivered at term. When offspring were weaned, they were placed on either CTR or HF diet for a period of 5 mo to yield four exposure models (in utero diet/postweaning diet: CTR/CTR n = 5; CTR/HF n = 4; HF/CTR n = 4; and HF/HF n = 5). Liver specimens were obtained at necropsy at 1 y of age.<br />Results: Hepatic trimethylation of lysine 4 of histone H3 is decreased (CTR/HF 0.87-fold, P = 0.038; HF/CTR 0.84-fold, P = 0.038), whereas hepatic methyltransferase activity increased by virtue of diet exposure (HF/HF 1.3-fold, P = 0.019). Using chromatin immunoprecipitation to determine Npas2 promoter occupancy, we found alterations of both repressive and permissive histone modifications specifically with postweaning HF diet exposure.<br />Conclusion: We found that altered Npas2 expression corresponds with a change in the histone code within the Npas2 promoter.
- Subjects :
- Animals
Blotting, Western
Chromatin Immunoprecipitation
Female
Histone Deacetylases metabolism
Histones metabolism
Macaca
Nerve Tissue Proteins metabolism
Pregnancy
DNA Methylation drug effects
Diet, High-Fat adverse effects
Histone Code drug effects
Liver metabolism
Prenatal Exposure Delayed Effects
Subjects
Details
- Language :
- English
- ISSN :
- 1530-0447
- Volume :
- 74
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Pediatric research
- Publication Type :
- Academic Journal
- Accession number :
- 23788059
- Full Text :
- https://doi.org/10.1038/pr.2013.106