Back to Search
Start Over
HLA typing by sequence-specific primers.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 1034, pp. 147-59. - Publication Year :
- 2013
-
Abstract
- HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1034
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 23775736
- Full Text :
- https://doi.org/10.1007/978-1-62703-493-7_8