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Retention of structure, antigenicity, and biological function of pneumococcal surface protein A (PspA) released from polyanhydride nanoparticles.
- Source :
-
Acta biomaterialia [Acta Biomater] 2013 Sep; Vol. 9 (9), pp. 8262-71. Date of Electronic Publication: 2013 Jun 14. - Publication Year :
- 2013
-
Abstract
- Pneumococcal surface protein A (PspA) is a choline-binding protein which is a virulence factor found on the surface of all Streptococcus pneumoniae strains. Vaccination with PspA has been shown to be protective against a lethal challenge with S. pneumoniae, making it a promising immunogen for use in vaccines. Herein the design of a PspA-based subunit vaccine using polyanhydride nanoparticles as a delivery platform is described. Nanoparticles based on sebacic acid (SA), 1,6-bis-(p-carboxyphenoxy)hexane (CPH) and 1,8-bis-(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG), specifically 50:50 CPTEG:CPH and 20:80 CPH:SA, were used to encapsulate and release PspA. The protein released from the nanoparticle formulations retained its primary and secondary structure as well as its antigenicity. The released PspA was also biologically functional based on its ability to bind to apolactoferrin and prevent its bactericidal activity against Escherichia coli. When the PspA nanoparticle formulations were administered subcutaneously to mice they elicited a high titer and high avidity anti-PspA antibody response. Together these studies provide a framework for the rational design of a vaccine against S. pneumoniae based on polyanhydride nanoparticles.<br /> (Copyright © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antigens, Bacterial administration & dosage
Antigens, Bacterial chemistry
Antigens, Bacterial immunology
Bacterial Proteins chemistry
Bacterial Vaccines administration & dosage
Bacterial Vaccines chemical synthesis
Bacterial Vaccines immunology
Delayed-Action Preparations administration & dosage
Diffusion
Materials Testing
Mice
Nanocapsules administration & dosage
Nanocapsules ultrastructure
Particle Size
Protein Conformation
Streptococcus pneumoniae drug effects
Bacterial Proteins administration & dosage
Bacterial Proteins immunology
Decanoic Acids chemistry
Delayed-Action Preparations chemical synthesis
Nanocapsules chemistry
Polyanhydrides chemistry
Polyesters chemistry
Streptococcus pneumoniae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-7568
- Volume :
- 9
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Acta biomaterialia
- Publication Type :
- Academic Journal
- Accession number :
- 23774257
- Full Text :
- https://doi.org/10.1016/j.actbio.2013.06.006